| 产品详细信息Description: The 53-7.3 monoclonal antibody reacts with mouse CD5, a 67 kDa protein expressed by a majority of thymocytes, mature T cells and a subset of B cells. The expression of CD5 by a small subset of B cells characterizes a developmentally and functionally distinct lineage of B cells called B-1 cells. CD5 is a counter-receptor for CD72 and plays a role in the T-B cell interaction.Applications Reported: This 53-7.3 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 53-7.3 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD5 is a 67 kDa human T-lymphocyte single-chain transmembrane glycoprotein. CD5 is present on all mature T-lymphocytes, on most of thymocytes and on many T-cell leukemias and lymphomas. CD5 also reacts with a subpopulation of activated B-cells and may act as a receptor in regulating T-cell proliferation. CD5 is found on 95% of thymocytes and 72% of peripheral blood lymphocytes. In lymph nodes, the main reactivity is observed in T cell areas. CD5 is expressed by many T cell leukemia, lymphomas, and activated T cells. Diseases associated with CD5 dysfunction include thymus cancer and Richter's Syndrome. |
| 1.Nature medicineThe creatine kinase pathway is a metabolic vulnerability in EVI1-positive acute myeloid leukemia."17-0051 was used in Flow cytometry/Cell sorting to investigate the effect of MECOM on cellular metabolism."AuthorsFenouille N,Bassil CF,Ben-Sahra I,Benajiba L,Alexe G,Ramos A,Pikman Y,Conway AS,Burgess MR,Li Q,Luciano F,Auberger P,Galinsky I,DeAngelo DJ,Stone RM,Zhang Y,Perkins AS,Shannon K,Hemann MT,Puissant A,Stegmaier K2.International immunologyEnhanced auto-antibody production and Mott cell formation in FcμR-deficient autoimmune mice."17-0051 was used in Flow cytometry/Cell sorting to determine the impact of Fcmr ablation on autoimmunity."AuthorsHonjo K,Kubagawa Y,Suzuki Y,Takagi M,Ohno H,Bucy RP,Izui S,Kubagawa H3.PloS oneNaïve helper T cells with high CD5 expression have increased calcium signaling."Published figure using CD5 monoclonal antibody (Product # 17-0051-82) in Flow Cytometry"4.PloS oneNaïve helper T cells with high CD5 expression have increased calcium signaling."Published figure using CD5 monoclonal antibody (Product # 17-0051-82) in Flow Cytometry"5.Nature communicationsNFAT2 is a critical regulator of the anergic phenotype in chronic lymphocytic leukaemia."17-0051 was used in Flow cytometry/Cell sorting to identify NFAT2 as a crucial regulator of the anergic phenotype in chronic lymphocytic leukaemia."AuthorsMärklin M,Heitmann JS,Fuchs AR,Truckenmüller FM,Gutknecht M,Bugl S,Saur SJ,Lazarus J,Kohlhofer U,Quintanilla-Martinez L,Rammensee HG,Salih HR,Kopp HG,Haap M,Kirschniak A,Kanz L,Rao A,Wirths S,Müller MR |
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