DyLight Antibody Labeling Kits/DyLight抗体标记试剂盒
货号：62275
规格：3 reactions
价格：6148
产品类型：蛋白标记
品牌：Thermo Fisher
| Invitrogen DyLight Antibody Labeling kits enable the creation of fluorescent antibodies that are covalently linked to different DyLight fluorophores. The fluorescently labeled antibodies can be used in a wide variety of laboratory applications, including immunofluorescence microscopy, IHC, western blotting, ELISA, flow cytometry, orin vitroandin vivofluorescent detection strategies.DyLight Antibody Labeling kits contain an NHS ester-activated derivative of the high-performance DyLight dye that can be used to fluorescently label antibodies and other proteins for cellular imaging and other fluorescence detection methods. The DyLight dye is an amine-reactive dye and is activated with an N-hydroxysuccinimide (NHS) ester moiety to react with exposed N-terminal α-amino groups or the ε-amino groups of lysine residues to form stable amide bonds.DyLight Antibody Labeling kits contain all necessary components to perform three separate labeling reactions and free dye removal using 1 mg of IgG or similar quantities of other proteins.DyLight dyes exhibit higher fluorescence intensity and photostability than CyDye and LI-COR dyes in many applications and remain highly fluorescent over a broad pH range (pH 4–9). Additionally, the water solubility of the DyLight fluorescent dyes allows a high dye-to-protein ratio to be achieved without causing precipitation of conjugates. Finally, the high solubility of these fluorophores permits protein solutions to be added directly to specific amounts of the labeling reagent.We also offer DyLight Microscale Antibody Labeling kits for fast and efficient fluorescent labeling of antibodies for use in fluorescence methods. The DyLight Microscale Antibody Labeling kits contain all necessary components to perform five separate labeling reactions using 100 µg of IgG or similar quantities of other proteins. Both the standard and microscale kit sizes include the amine-reactive DyLight fluorophore in convenient single-use vials as well as purification resin and spin columns for the preparation of ready-to-use conjugate. |
| 特点 |
| · High performance—high fluorescence intensity across a broad pH range· Multiple colors—10 different dyes across the visible spectrum and extending into the infrared· Specificity—NHS ester-activated dye labels proteins and other molecules at primary amines (-NH2)· Optimized procedure—following the standard protocol results in antibodies with excellent dye:protein ratios and recovery rates for optimum activity and fluorescence labeling |
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