Pierce NHS-Rhodamine Antibody Labeling Kit/Pierce NHS-若丹明抗体标记试剂盒
货号：53031
规格：3 reactions
价格：6271
产品类型：蛋白标记
品牌：Thermo Fisher
| Thermo Scientific NHS-Rhodamine is a high-performance derivative of rhodamine dye, activated for easy and reliable labeling of antibodies, proteins and other molecules for use as fluorescent probes. Use our NHS-Rhodamine Antibody Labeling Kit to label and purify antibodies in about one hour.NHS Rhodamine is an amine-reactive derivative of rhodamine dye that has wide-ranging application as antibody and other probe labels for use in fluorescence microscopy, flow cytometry and immunofluorescence-based assays such as western blotting and ELISA.Properties of Rhodamine Dyes:Thermo Scientific Pierce Rhodamine Dyes are mixtures of isomers with reactive groups attached at the 5- and 6-positions of the bottom ring. The properties of these isomers are indistinguishable in terms of excitation and emission spectra, and for protein applications there is no need to isolate a specific isomer.NHS-Rhodamine is activated with the N-hydroxy-succinimidyl-ester (NHS ester) functional group. Compared to TRITC, the NHS-ester deriviative has greater specificity toward primary amines in the presence of other nucleophiles and results in a more stable linkage following labeling. |
| 特点 |
| •Amine-specific labeling—NHS-ester varieties of rhodamine efficiently label antibodies and other purified proteins at primary amines (lysine side chains)•Optimized procedure—following the standard protocol results in antibodies with excellent dye:protein ratios for optimum activity and fluorescence•Single-use fluors—no need to weigh tiny amounts powder; kit contains single-use vials of reagent•Efficient purification—kit includes purification resin and easy-to-use spin columns, ensuring rapid and efficient removal of non-reacted dye and excellent protein recovery |
| 应用 |
| • Label antibodies for use as immunofluorescent probes• Label oligonucleotides for hybridization probes• Detect proteins in gels and on western blots |
| 数据 |
Detection of α-tubulin in A549 cells demonstrates use of rhodamine-labeled secondary antibodyCells were grown in 96-well microplates for 18-20 hrs, fixed with 4% paraformaldehyde (Part No. 28906) and permeabilized with 0.1% Surfact-Amps X-100 (Part No. 28314). Cells were then probed with a mouse anti-α-tubulin primary antibody (0.4µg/mL) and Rhodamine-goat anti-mouse secondary antibody (2µg/mL). Nuclei were labeled with Hoechst Dye. Images were acquired by fluorescence microscopy.A.Fluorescence image shows a delicate network of α-tubulin (pseudo-colored green) located exclusively in the cytoplasm.B.Nuclear counterstain with Hoechst Dye (pseudo-colored blue)C.Merged image. Comparison of TRITC and rhodamine dyes |
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