| 抗体类型 | 单抗 | 同型对照： | Mouse IgG2b kappa Isotype Control (eBMG2b), Alexa Fluor 488 |
| 浓度： | 5 µL/Test | 用法： | 5 µL (0.125 µg)/test (Flow) |
| 产品详细信息Description: The OKT4 monoclonal antibody reacts with human CD4, a 59 kDa cell surface glycoprotein expressed by the majority of thymocytes, a subpopulation of mature T cells (T-helper cells) and in low levels on monocytes. CD4 is a receptor for the human immunodeficiency virus (HIV). The OKT4 antibody recognizes a different epitope than the RPA-T4 monoclonal antibody, and these antibodies do not cross-block binding to each other's respective epitopes.Applications Reported: This OKT4 (OKT-4) antibody has been reported for use in flow cytometric analysis.Applications Tested: This OKT4 (OKT-4) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息The CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV. CD4 is primarily expressed in a subset of T-lymphocytes, also referred to as T helper cells, but may also be expressed by other cells in the immune system, such as monocytes, macrophages, and dendritic cells. At the tissue level, CD4 expression may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues. CD4 functions to initiate or augment the early phase of T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase, Lck. It may also function as an important mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcripts have been identified in this gene [RefSeq, July 2017]. |
| 数据 |
| CD4 Antibody (53-0048-42)Nature communications 2019 -Fig. 1 CD20 dim CD4 + T cells involve memory phenotypes with more activation than CD20 - cells. PBMC from uninfected donors, ART-suppressed patients and viremic patients were stained with CD20, activation markers (HLA-DR, CD69, CD25), and T-cell differentiation markers (CCR7, CD45RO, CD27, CD95). a Gating strategy used to identify CD20 dim CD4 + T cells. Previous sequential gates are represented in Supplementary Fig. 9 . Viable CD4 + T cells (identified by CD3 and CD4 expression) were first selected for the analysis of CD20 expression, defined by its Fluorescence Minus One (FMO) control. T-cell memory subsets were selected as follows: CD4 + T NA (CCR7 + , CD45RO - , CD27 + , CD95 - ), CD4 + T SCM (CCR7 + , CD45RO - , CD27 + , CD95 + ); CD4 + T CM (CCR7 + , CD45RO + ), CD4 + T TM (CCR7 - , CD45RO + , CD27 + ); CD4 + T EM (CCR7 - , CD45RO + , CD27 - ), CD4 + T TD (CCR7 - , CD45RO - ). b , c Representative bright-field and pseudo-color fluorescence images of CD20 dim CD4 + T cells ( b ) and B cells ( c ) from two ART-suppressed patients (#9 and #22) using the Amnis imaging flow cytometer technology. Scale bar 10 um. d Percentage of CD20 dim expression within CD4 + T cells in uninfected controls and the two patient cohorts. Mann-Whitney comparison was used to compare n = 6 uninfected controls, n = 21 ART-suppressed patients, n = 20 viremic patients. e Expression of the activation markers CD25, CD69 or HLA-DR in CD20 dim and CD20 - CD4 + T cells in different cohorts of HIV + patie |
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| 参考文献： |
| 1.BloodCD4-CCR5 interaction in intracellular compartments contributes to receptor expression at the cell surface."Published figure using CD4 monoclonal antibody (Product # 53-0048-42) in Flow Cytometry"2.PloS oneAn increased abundance of tumor-infiltrating regulatory T cells is correlated with the progression and prognosis of pancreatic ductal adenocarcinoma."Published figure using CD4 monoclonal antibody (Product # 53-0048-42) in Immunohistochemistry"3.BloodCD4-CCR5 interaction in intracellular compartments contributes to receptor expression at the cell surface."Published figure using CD4 monoclonal antibody (Product # 53-0048-42) in Flow Cytometry"4.Nature communicationsExpression of CD20 after viral reactivation renders HIV-reservoir cells susceptible to Rituximab."Published figure using CD4 monoclonal antibody (Product # 53-0048-42) in Flow Cytometry"5.Oncology lettersAccumulation of T-helper 22 cells, interleukin-22 and myeloid-derived suppressor cells promotes gastric cancer progression in elderly patients."Published figure using CD4 monoclonal antibody (Product # 53-0048-42) in Flow Cytometry" |
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