| Description:The OKT8 monoclonal antibody reacts with the human CD8a molecule, an approximately 32-34 kDa cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha beta) or as a homodimer (CD8 alphaalpha). A majority of thymocytes and a subpopulation of mature T cells and NK cells express CD8a. CD8 binds to MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T-cell development and activation of mature T cells. Preliminary testing indicates that OKT8 and two other mouse anti-human CD8 antibodies (clone RPA-T8, Cat. No.14-0088 and clone HIT8a, Cat. No.14-0089) do not compete with each other for binding to human peripheral blood leukocytes by flow cytometric analysis, suggesting that they do not bind to similar epitopes or block each other by steric hindrance.Applications Reported: This OKT8 (OKT-8) antibody has been reported for use in flow cytometric analysis.Applications Tested: This OKT8 (OKT-8) antibody is offered in 2 formats: - µg size: has been tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. - test size: has been pre-titrated and tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Alexa Fluor® 700 emits at 723 nm and can be excited with the red laser (633 nm). Most instruments will require a 685 LP mirror and 710/20 filter. Please make sure that your instrument is capable of detecting this fluorochrome.Excitation: 633-647 nm; Emission: 723 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.Target InformationCD8 molecule is composed of two chains termed alpha and beta. CD8 is found on a T cell subset of normal cytotoxic / suppressor cells which make up approximately 20 to 35% of human peripheral blood lymphocytes. The CD8 antigen is also detected on natural killer cells, 80% of thymocytes, on a subpopulation of 30% of peripheral blood null cells and 15 to 30% of bone marrow cells.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization. |
| 1. The Journal of biological chemistryStructure and Membrane Binding Properties of the Endosomal Tetratricopeptide Repeat (TPR) Domain-containing Sorting Nexins SNX20 and SNX21."Published figure using CD8a monoclonal antibody (Product # 56-0086-42) in Immunofluorescence"2. Frontiers in pharmacologyDisruption of PD-1 Enhanced the Anti-tumor Activity of Chimeric Antigen Receptor T Cells Against Hepatocellular Carcinoma."Published figure using CD8a monoclonal antibody (Product # 56-0086-42) in Flow Cytometry"3. NPJ precision oncologyTranscriptomic analysis of hepatocellular carcinoma reveals molecular features of disease progression and tumor immune biology."56-0086 was used in Flow cytometry/Cell sorting to identify unique molecular signatures pertaining to hepatocellular carcinoma disease progression and tumour immunity."AuthorsOkrah K,Tarighat S,Liu B,Koeppen H,Wagle MC,Cheng G,Sun C,Dey A,Chang MT,Sumiyoshi T,Mounir Z,Cummings C,Hampton G,Amler L,Fridlyand J,Hegde PS,Turley SJ,Lackner MR,Huang SM4. Cancer immunology researchPhase I Trial of a Yeast-Based Therapeutic Cancer Vaccine (GI-6301) Targeting the Transcription Factor Brachyury."56-0086 was used in Immuno-assay to investigate the effect of GI-6301 on Brachyury in clinical trials."AuthorsHeery CR,Singh BH,Rauckhorst M,Marté JL,Donahue RN,Grenga I,Rodell TC,Dahut W,Arlen PM,Madan RA,Schlom J,Gulley JL |
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