| 产品详细信息Description: The OKT4 monoclonal antibody reacts with human CD4, a 59 kDa cell surface glycoprotein expressed by the majority of thymocytes, a subpopulation of mature T cells (T-helper cells) and in low levels on monocytes. CD4 is a receptor for the human immunodeficiency virus (HIV). The OKT4 antibody recognizes a different epitope than the RPA-T4 monoclonal antibody, and these antibodies do not cross-block binding to each other's respective epitopes.Applications Reported: This OKT4 (OKT-4) antibody has been reported for use in flow cytometric analysis.Applications Tested: This OKT4 (OKT-4) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息The CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV. CD4 is primarily expressed in a subset of T-lymphocytes, also referred to as T helper cells, but may also be expressed by other cells in the immune system, such as monocytes, macrophages, and dendritic cells. At the tissue level, CD4 expression may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues. CD4 functions to initiate or augment the early phase of T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase, Lck. It may also function as an important mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcripts have been identified in this gene [RefSeq, July 2017]. |
| 1.Immunology and cell biologyMemory CD8+ T cells from naturally acquired primary dengue virus infection are highly cross-reactive."48-0048 was used in Flow cytometry/Cell sorting to demonstrate significant DENV serotype-cross-reactivity of CD8+ T-cells after naturally-acquired primary infection."AuthorsFriberg H,Burns L,Woda M,Kalayanarooj S,Endy TP,Stephens HA,Green S,Rothman AL,Mathew A2.Cancer cellEnhanced Anti-lymphoma Activity of CAR19-iNKT Cells Underpinned by Dual CD19 and CD1d Targeting."48-0048 was used in Flow cytometry/Cell sorting to investigate whether chimeric antigen receptor anti-CD19-engineering of the CD1d-restricted invariant natural killer T cells would result in enhanced anti-lymphoma activity."AuthorsRotolo A,Caputo VS,Holubova M,Baxan N,Dubois O,Chaudhry MS,Xiao X,Goudevenou K,Pitcher DS,Petevi K,Kachramanoglou C,Iles S,Naresh K,Maher J,Karadimitris A3.PloS oneA Comprehensive Analysis of the Impact of HIV on HCV Immune Responses and Its Association with Liver Disease Progression in a Unique Plasma Donor Cohort."48-0048 was used in Flow cytometry/Cell sorting to highlights the impact of HIV co-infection on HCV-specific CD4+ T-cell responses."AuthorsZhang YH,Zhao Y,Rajapaksa US,Lawrence TM,Peng YC,Liu J,Xu K,Hu K,Qin L,Liu N,Sun H,Yan HP,Repapi E,Rowland-Jones S,Thimme R,McKeating JA,Dong T4.Journal of immunology (Baltimore, Md. : 1950)Influenza Vaccination Generates Cytokine-Induced Memory-like NK Cells: Impact of Human Cytomegalovirus Infection."48-0048 was used in Flow cytometry/Cell sorting to examine the effect of seasonal influenza vaccination on natural killer cell function and phenotype in previously unvaccinated individuals."AuthorsGoodier MR,Rodriguez-Galan A,Lusa C,Nielsen CM,Darboe A,Moldoveanu AL,White MJ,Behrens R,Riley EM5.Cancer biology and therapyDurable complete responses off all treatment in patients with metastatic malignant melanoma after sequential immunotherapy followed by a finite course of BRAF inhibitor therapy."48-0048 was used in Flow cytometry/Cell sorting to study the effectiveness of different therapy regimes on treating melanoma after failure of immunotherapy."AuthorsWyluda EJ,Cheng J,Schell TD,Haley JS,Mallon C,Neves RI,Robertson G,Sivik J,Mackley H,Talamo G,Drabick JJ |
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