CD4 Monoclonal Antibody (OKT4 (OKT-4)), PE-Cyanine7
货号：25-0048-41,25-0048-42
规格：25 Tests,100 Tests
价格：1215,3035
产品类型：流式抗体
品牌：Thermo Fisher
物种：人
宿主：小鼠
抗体亚型：IgG2b, kappa
荧光染料：PE-Cy7
| 抗体类型 | 单抗 | 同型对照： | Mouse IgG2b kappa Isotype Control (eBMG2b), PE-Cyanine7 |
| 浓度： | 5 µL/Test | 用法： | 5 μL (0.125 μg)/test(Flow) |
| 产品详细信息Description: The OKT4 monoclonal antibody reacts with human CD4, a 59 kDa cell surface glycoprotein expressed by the majority of thymocytes, a subpopulation of mature T cells (T-helper cells) and in low levels on monocytes. CD4 is a receptor for the human immunodeficiency virus (HIV). The OKT4 antibody recognizes a different epitope than the RPA-T4 monoclonal antibody, and these antibodies do not cross-block binding to each other's respective epitopes.Applications Reported: This OKT4 (OKT-4) antibody has been reported for use in flow cytometric analysis.Applications Tested: This OKT4 (OKT-4) antibody has been pre-titrated and tested by flow cytometric analysis of normal human blood peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息The CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV. CD4 is primarily expressed in a subset of T-lymphocytes, also referred to as T helper cells, but may also be expressed by other cells in the immune system, such as monocytes, macrophages, and dendritic cells. At the tissue level, CD4 expression may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues. CD4 functions to initiate or augment the early phase of T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase, Lck. It may also function as an important mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcripts have been identified in this gene [RefSeq, July 2017]. |
| 数据 |
| CD4 Antibody (25-0048-41) in FlowStaining of normal human peripheral blood cells with Anti-Human CD19 eFluor® 450 (Product # 48-0199-42) and Mouse IgG2b K Isotype Control PE-Cyanine7 (Product # 25-4732-81) (left) or Anti-Human CD4 PE-Cyanine7 (right). Cells in the lymphocyte gate were used for analysis. |
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| 参考文献： |
| 1.Nature communicationsIL-6 receptor blockade corrects defects of XIAP-deficient regulatory T cells."Published figure using CD4 monoclonal antibody (Product # 25-0048-42) in Flow Cytometry"2.Scientific reportsIntracellular delivery of mRNA to human primary T cells with microfluidic vortex shedding."25-0048 was used in Flow cytometry/Cell sorting to show microfluidic vortex shedding mediates intracellular delivery of mRNA into primary human T lymphocytes."AuthorsJarrell JA,Twite AA,Lau KHWJ,Kashani MN,Lievano AA,Acevedo J,Priest C,Nieva J,Gottlieb D,Pawell RS3.Neuro-oncologyPD-L1 expression and prognostic impact in glioblastoma."Published figure using CD4 monoclonal antibody (Product # 25-0048-42) in Flow Cytometry"4.Nature communicationsIL-6 receptor blockade corrects defects of XIAP-deficient regulatory T cells."Published figure using CD4 monoclonal antibody (Product # 25-0048-42) in Flow Cytometry"5.Immunity, inflammation and diseaseCorrelation of APRIL with production of inflammatory cytokines during acute malaria in the Brazilian Amazon.AuthorsPinna RA,Dos Santos AC,Perce-da-Silva DS,da Silva LA,da Silva RNR,Alves MR,Santos F,de Oliveira Ferreira J,Lima-Junior JC,Villa-Verde DM,De Luca PM,Carvalho-Pinto CE,Banic DM |
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