CD8a Monoclonal Antibody (SK1), APC-eFluor 780, eBioscience™

货号：47-0087-41,47-0087-42

规格：25tests,100tests

价格：1316,3035

产品类型：流式抗体

品牌：Thermo Fisher

抗原：CD8a

物种：人

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：SK1

荧光染料：APC-eFluor 780

抗体类型:	流式抗体	同型对照：	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), APC-eFluor 780, eBioscience
浓度：	5 µL/Test	用法：	5 µL (0.5 µg)/test (Flow）

产品详细信息Description:The SK1 monoclonal antibody reacts with the human CD8a molecule, an approximately 32-34 kDa cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha/beta) or as a homodimer (CD8 alpha/alpha). A majority of thymocytes and a subpopulation of mature T cells and NK cells express CD8a. CD8 binds to MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T-cell development and activation of mature T cells.Applications Reported: This SK1 (SK-1) antibody has been reported for use in flow cytometric analysis.Applications Tested: This SK1 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.APC-eFluor 780 emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.Light sensitivity: This tandem is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.Target InformationCD8 molecule is composed of two chains termed alpha and beta. CD8 is found on a T cell subset of normal cytotoxic / suppressor cells which make up approximately 20 to 35% of human peripheral blood lymphocytes. The CD8 antigen is also detected on natural killer cells, 80% of thymocytes, on a subpopulation of 30% of peripheral blood null cells and 15 to 30% of bone marrow cells.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

CD8a Antibody (47-0087-41) in FlowStaining of normal human peripheral blood cells with Anti-Human CD3 FITC (Product # 11-0038-42) and Mouse IgG1 K Isotype Control APC-eFluor® 780 (Product # 47-4714-82) (left) or Anti-Human CD8a APC-eFluor® 780 (right). Cells in the lymphocyte gate were used for analysis.

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参考文献：

1.Arthritis research and therapyImpaired lymph node stromal cell function during the earliest phases of rheumatoid arthritis.2.mBioHigh Prevalence of MERS-CoV Infection in Camel Workers in Saudi Arabia."47-0087 was used in Flow cytometry/Cell sorting to show that approximately 50% of camel workers in the Kingdom of Saudi Arabia and 0% of controls were previously infected."3.Nature medicineSNP-mediated disruption of CTCF binding at the IFITM3 promoter is associated with risk of severe influenza in humans. "47-0087 was used in Flow cytometry/Cell sorting to identify a novel regulator of IFITM3 expression that associates with CD8 T cell levels in the airways and a spectrum of clinical outcomes."

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