CD8a Monoclonal Antibody (SK1), PerCP-eFluor 710, eBioscience™

货号：46-0087-41,46-0087-42

规格：25tests,100tests

价格：1754,3331

产品类型：流式抗体

品牌：Thermo Fisher

物种：人

宿主：小鼠

抗体亚型：IgG1, kappa

荧光染料：PerCP-eFluor™ 710

抗体类型:	流式抗体	同型对照：	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PerCP-eFluor 710, eBioscience
浓度：	5 µL/Test	用法：	5 µL (0.125 µg)/test (Flow）

产品详细信息Description:The SK1 monoclonal antibody reacts with the human CD8a molecule, an approximately 32-34 kDa cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha/beta) or as a homodimer (CD8 alpha/alpha). A majority of thymocytes and a subpopulation of mature T cells and NK cells express CD8a. CD8 binds to MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T-cell development and activation of mature T cells.Applications Reported: This SK1 (SK-1) antibody has been reported for use in flow cytometric analysis.Applications Tested: This SK1 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.Target InformationCD8 molecule is composed of two chains termed alpha and beta. CD8 is found on a T cell subset of normal cytotoxic / suppressor cells which make up approximately 20 to 35% of human peripheral blood lymphocytes. The CD8 antigen is also detected on natural killer cells, 80% of thymocytes, on a subpopulation of 30% of peripheral blood null cells and 15 to 30% of bone marrow cells.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

v CD8a Antibody (46-0087-41) in FlowStaining of normal human peripheral blood cells with Anti-Human CD19 APC (Product # 17-0199-42) and Mouse IgG1 K Isotype Control PerCP-eFluor® 710 (Product # 46-4714-82) (left) or Anti-Human CD8a PerCP-eFluor® 710 (right). Cells in the lymphocyte gate were used for analysis.

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参考文献：

1.PLoS pathogensIn vivo activation of latent HIV with a synthetic bryostatin analog effects both latent cell "kick" and "kill" in strategy for virus eradication."46-0087 was used in Flow cytometry/Cell sorting to show that cells latently-infected by HIV exhibit both "kick" and "kill" strategies upon virus reactivation with a bryostatin 1 analogue."

技术参数

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