CD45RA Monoclonal Antibody (HI100), eFluor 450, eBioscience™

货号：48-0458-42,48-0458-41

规格：100 Tests,25 Tests

价格：2820,1151

产品类型：流式抗体

品牌：eBioscience

抗原：CD45RA

物种：人

宿主：小鼠

抗体亚型：IgG2b, kappa

克隆号：HI100

荧光染料：eFluor 450

类型:	一抗	同型对照：	Mouse IgG2b kappa Isotype Control (eBMG2b), eFluor 450, eBioscience™
浓度：	5 µL/Test	用法：	5 µL (0.5 µg)/test（Flow）

产品详细信息

Description: The HI100 monoclonal antibody reacts with human CD45RA, a 220 kDa molecule expressed by subpopulations of CD4+ peripheral T lymphocytes, CD8+ peripheral T lymphocytes, and B cells. The CD45RA+ T cell populations are mainly naive/virgin allowing the use of HI100 mAb as a phenotypic marker to discriminate T cell subsets.Applications Reported: This HI100 antibody has been reported for use in flow cytometric analysis.Applications Tested: This HI100 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral mononuclear cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing and these isoforms differ in their extracellular domains, however, they share identical transmembrane and cytoplasmic domains. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45 is expressed on cells of the human hematopoietic lineage, with the exception of mature red cells and is not detected on differentiated cells of other tissues. It is likely that CD45 plays an important role in signal transduction, inhibition or upregulation of various immunological functions. Diseases associated with CD45 dysfunction include severe combined immunodeficiency and Hepatitis C.仅用于科研。不用于诊断过程。

数据

CD45RA Antibody (48-0458-42) in FlowStaining of normal human peripheral blood cells with Anti-Human CD45RO FITC (Product # 11-0457-42) and Mouse IgG2b K Isotype Control eFluor® 450 (Product # 48-4732-82) (left) or Anti-Human CD45RA eFluor® 450 (right). Cells in the lymphocyte gate were used for analysis.

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参考文献：

1.Journal of immunology researchAutomatic Generation of Validated Specific Epitope Sets."48-0458 was used in Flow cytometry/Cell sorting to develop a method to automatically select reference epitope sets according to a categorization system employed by the IEDB."AuthorsCarrasco Pro S,Sidney J,Paul S,Lindestam Arlehamn C,Weiskopf D,Peters B,Sette A2.Journal of immunology (Baltimore, Md. : 1950)Type 1 diabetes-associated IL2RA variation lowers IL-2 signaling and contributes to diminished CD4+CD25+ regulatory T cell function."48-0458 was used in Flow cytometry/Cell sorting to explore IL2RA gene polymorphisms in Treg dysfunction and associations with autoimmune and inflammatory diseases."AuthorsGarg G,Tyler JR,Yang JH,Cutler AJ,Downes K,Pekalski M,Bell GL,Nutland S,Peakman M,Todd JA,Wicker LS,Tree TI3.Nature communicationsTargeting enhancer switching overcomes non-genetic drug resistance in acute myeloid leukaemia."Published figure using CD45RA monoclonal antibody (Product # 48-0458-42) in Flow Cytometry"4.Journal of virologyImmunodominant Dengue Virus-Specific CD8+ T Cell Responses Are Associated with a Memory PD-1+ Phenotype."48-0458 was used in Flow cytometry/Cell sorting to define DENV-specific CD8(+)T cell responses in general and those of HLA alleles associated with dominant responses."Authorsde Alwis R,Bangs DJ,Angelo MA,Cerpas C,Fernando A,Sidney J,Peters B,Gresh L,Balmaseda A,de Silva AD,Harris E,Sette A,Weiskopf D5.Journal of virologyThe human CD8+ T cell responses induced by a live attenuated tetravalent dengue vaccine are directed against highly conserved epitopes."48-0458 was used in Flow cytometry/Cell sorting to characterise the CD8(+) T cell responses after live attenuated dengue vaccination."AuthorsWeiskopf D,Angelo MA,Bangs DJ,Sidney J,Paul S,Peters B,de Silva AD,Lindow JC,Diehl SA,Whitehead S,Durbin A,Kirkpatrick B,Sette A

技术参数

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