CD7 Monoclonal Antibody (4H9), PerCP-eFluor 710, eBioscience™

货号：46-0078-41,46-0078-42

规格：25 Tests,100 Tests

价格：1752,3615

产品类型：流式抗体

品牌：eBioscience

抗原：CD7

物种：人

宿主：小鼠

抗体亚型：IgG2a, kappa

克隆号：4H9

荧光染料：PerCP-eFluor™ 710

类型:	一抗	同型对照：	Mouse IgG2a kappa Isotype Control (eBM2a), PerCP-eFluor 710, eBioscience™
浓度：	5 µL/Test	用法：	5 µL (0.25 µg)/test（Flow）

产品详细信息Description: This 4H9 monoclonal antibody reacts with human CD7 (also known as Leu-9 or gp40), a 40-kDa transmembrane glycoprotein belonging to the immunoglobulin superfamily. This molecule is expressed on peripheral blood T lymphocytes and natural killer cells, as well as on lymphoid and myeloid lineage precursors during adult hematopoiesis. Capable of providing a costimulatory signal and associating with CD3 and CD45, CD7 is involved in T cell activation.CD7 has also been detected on numerous T cell-derived leukemias and lymphomias. In fact, because CD7 expression is greater on CD4+ T cells in classical Hodgkin's lymphoma than in benign reactive lymph adenopathy, this molecule may be used as a diagnostic for Hodgkin's lymphoma.Blocking assays demonstrate that this clone recognizes the same epitope as clone 124-1D1.Applications Reported: This 4H9 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 4H9 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD7 (gp40, Leu9) is a 40 kDa member of the immunoglobulin gene superfamily. CD7 contains N-terminal amino acids 1-107 are highly homologous to Ig kappa-L chains whereas the carboxy-terminal region of the extracellular domain is proline-rich and has been postulated to form a stalk from which the Ig domain projects. CD7 is expressed on the majority of immature and mature T-lymphocytes, and T cell leukemia. Further, CD7 is also found on natural killer cells, a small subpopulation of normal B cellsand on malignant B cells. Cross-linking surface CD7 positively modulates T cell and NK cell activity as measured by calcium fluxes, expression of adhesion molecules, cytokine secretion and proliferation. CD7 associates directly with phosphoinositol 3'-kinase. CD7 ligation induces production of D-3 phosphoinositides and tyrosine phosphorylation. Expression of CD7 is an important marker used in leukemia diagnostics.仅用于科研。不用于诊断过程。

数据

CD7 Antibody (46-0078-41) in FlowStaining of normal human peripheral blood cells with Anti-Human CD4 PE (Product # 12-0048-42) and Mouse IgG2a K Isotype Control PerCP-eFluor® 710 (Product # 46-4724-82) (left) or Anti-Human CD7 PerCP-eFluor® 710 (right). Cells in the lymphocyte gate were used for analysis.

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