CD7 Monoclonal Antibody (eBio124-1D1 (124-1D1)), Alexa Fluor 700, eBioscience™

货号：56-0079-42

规格：100 Tests

价格：2732

产品类型：流式抗体

品牌：eBioscience

抗原：CD7

物种：人

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：eBio124-1D1 (124-1D1)

荧光染料：Alexa Fluor 700

类型:	一抗	同型对照：	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Alexa Fluor 700, eBioscience™
浓度：	5 µL/Test	用法：	5 µL (0.5 µg)/test（Flow）

产品详细信息Description: The eBio124-1D1 monoclonal antibody reacts with human CD7, also known as gp40 and Leu9. CD7, a 40 kD receptor, is a member of the immunoglobulin gene superfamily. The N-terminal amino acid sequence (aa1-107) is highly homologous to Ig kappa light chain sequence; while the carboxyl-terminal region of the extracellular domain is proline-rich and has been postulated to form a stalk from which the Ig domain projects. CD7 is expressed on the majority of immature and mature T lymphocytes,and T cell leukemias. It is also found on natural killer cells, a small suppopulation of normal B cells and on maligant B cells. Cross-linking surface CD7 positively modulates T cell and NK cell activity, as measured by calcium flux, expression of adhesion molecules, cytokine secretion and proliferation. CD7 associates directly with phosphoinositol 3'-kinase. CD7 ligation induces production of D-3 phosphoinositides and tyrosine phosphorylation.A clonogenic subpopulation of human CD34(+) CD38(-) cord blood cells that express CD45RA and HLA-DR and high levels of the CD7 has been reported. These cells possess the capacity for lymphopoiesis. They can generate B-cells, natural killer cells, and dendritic cells but do not possess the capacity to develop into myeloid cells or erythroid cells. The CD7(+) phenotype distinguishes primitive human lymphoid progenitors from pluripotent stem cells.Furthermore, it has been suggested that CD7 co-operates with CD28 during Treg function, as mice deficient in both CD28 and CD7 have reduced total numbers of Tregs and these Tregs have reduced suppressive activity.Applications Reported: This eBio124-1D1 (124-1D1) antibody has been reported for use in flow cytometric analysis.Applications Tested: This eBio124-1D1 (124-1D1) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Alexa Fluor® 700 emits at 723 nm and can be excited with the red laser (633 nm). Most instruments will require a 685 LP mirror and 710/20 filter. Please make sure that your instrument is capable of detecting this fluorochrome.Excitation: 633-647 nm; Emission: 723 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD7 (gp40, Leu9) is a 40 kDa member of the immunoglobulin gene superfamily. CD7 contains N-terminal amino acids 1-107 are highly homologous to Ig kappa-L chains whereas the carboxy-terminal region of the extracellular domain is proline-rich and has been postulated to form a stalk from which the Ig domain projects. CD7 is expressed on the majority of immature and mature T-lymphocytes, and T cell leukemia. Further, CD7 is also found on natural killer cells, a small subpopulation of normal B cellsand on malignant B cells. Cross-linking surface CD7 positively modulates T cell and NK cell activity as measured by calcium fluxes, expression of adhesion molecules, cytokine secretion and proliferation. CD7 associates directly with phosphoinositol 3'-kinase. CD7 ligation induces production of D-3 phosphoinositides and tyrosine phosphorylation. Expression of CD7 is an important marker used in leukemia diagnostics.仅用于科研。不用于诊断过程。

数据

CD7 Antibody (56-0079-42) in FlowStaining of normal human peripheral blood cells with Anti-Human CD3 FITC (Product # 11-0038) and Mouse IgG1 K Isotype Control Alexa Fluor® 700 (Product # 56-4714) (left) or Anti-Human CD7 Alexa Fluor® 700 (right). Cells in the lymphocyte gate were used for analysis.

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参考文献：

1.Bio-protocolEx vivoHuman Natural Killer (NK) Cell Stimulation and Intracellular IFNγ and CD107a Cytokine Staining."56-0079 was used in Flow cytometry/Cell sorting to describe a method to assess IFNγ and CD107a expression in NK cells following stimulation with target cells or cytokines."AuthorsYork VA,Milush JM2.Stem cell reportsTargeted Disruption of TCF12 Reveals HEB as Essential in Human Mesodermal Specification and Hematopoiesis."56-0079 was used in Flow cytometry/Cell sorting to identify HEB as a critical regulator of human mesodermal and hematopoietic specification."3.JCI insightElderly human hematopoietic progenitor cells express cellular senescence markers and are more susceptible to pyroptosis."56-0079 was used in Flow cytometry/Cell sorting to study blood haematopoietic progenitor cells from older humans."AuthorsFali T,Fabre-Mersseman V,Yamamoto T,Bayard C,Papagno L,Fastenackels S,Zoorab R,Koup RA,Boddaert J,Sauce D,Appay V

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