CD25 Monoclonal Antibody (BC96), Alexa Fluor 488, eBioscience™

货号：53-0259-41,53-0259-42

规格：25 Tests,100 Tests

价格：1011,3107

产品类型：流式抗体

品牌：eBioscience

抗原：CD25

物种：人

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：BC96

荧光染料：Alexa Fluor 488

类型:	一抗	同型对照：	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Alexa Fluor 488, eBioscience™
浓度：	5 µL/Test	用法：	5 µL (0.125 µg)/test（Flow）

产品详细信息Description: The BC96 monoclonal antibody reacts with human CD25, the 55 kDa interleukin-2 receptor alpha chain (IL-2Ralpha). CD25 is expressed by early progenitors of T and B lineage as well as by activated mature T and B lymphocytes. By itself, CD25 binds IL-2 only with low affinity. However, CD25 associates with CD122 (IL-2 receptor beta chain) and CD132 (common gamma chain) to form the high affinity IL-2 receptor. CD25 plays a role in lymphocyte differentiating and activation/proliferation.<br>Applications Reported: This BC96 antibody has been reported for use in flow cytometric analysis.Applications Tested: This BC96 antibody has been pre-titrated and tested by flow cytometric analysis of human normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.When staining for CD25 (with emission at 518nm) it is recommended to use Alexa Fluor 488 conjugated antibodies, rather than FITC for optimal detection.Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD25 (IL2 receptor alpha chain/IL2RA) is a cytokine that plays a role in the proliferation of T and B lymphocytes. The receptor of this cytokine (IL2RA) is a heterotrimeric protein complex with a gamma chain also shared by interleukin 4 (IL4) and interleukin 7 (IL7). IL2RA, IL2R beta chain (IL2RB), and the IL2R gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric IL2RA chains result in low-affinity receptor, while homodimeric IL2RB chains produce a medium-affinity receptor. The expression of IL2 in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. IL2 is primarily produced by mature T cells. IL2 plays an important role as a growth factor, differentiation factor, and regulator of cell death. IL-2 stimulates the proliferation of B cells, augments natural killer cell activity, and inhibits granulocyte macrophage colony formation. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests a role in the immune response to antigenic stimuli. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.仅用于科研。不用于诊断过程。

数据

CD25 Antibody (53-0259-41) in FlowStaining of normal human peripheral blood cells with Anti-Human CD4 Alexa Fluor® 647 and Mouse IgG1 kappa Isotype Control Alexa Fluor® 488 (Product # 53-4714-42) (left) or Anti-Human CD25 Alexa Fluor® 488 (right). Cells in the lymphocyte gate were used for analysis.

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参考文献：

1.Frontiers in pharmacologyDisruption of PD-1 Enhanced the Anti-tumor Activity of Chimeric Antigen Receptor T Cells Against Hepatocellular Carcinoma."Published figure using CD25 monoclonal antibody (Product # 53-0259-42) in Flow Cytometry"2.Experimental and therapeutic medicineEquilibrium of Treg/Th17 cells of peripheral blood in syphilitic patients with sero-resistance."Published figure using CD25 monoclonal antibody (Product # 53-0259-42) in Flow Cytometry"3.Scientific reportsCombinations of isoform-targeted histone deacetylase inhibitors and bryostatin analogues display remarkable potency to activate latent HIV without global T-cell activation."Published figure using CD25 monoclonal antibody (Product # 53-0259-42) in Flow Cytometry"4.Nature communicationsTGF-β3-expressing CD4+CD25(-)LAG3+ regulatory T cells control humoral immune responses."53-0259 was used in Flow cytometry/Cell sorting to investigate the role of GF-β3-expressing CD4+CD25(-)LAG3+ Tregs in humoral immune responses."AuthorsOkamura T,Sumitomo S,Morita K,Iwasaki Y,Inoue M,Nakachi S,Komai T,Shoda H,Miyazaki J,Fujio K,Yamamoto K5.BMC immunologyFunctional requirements for inhibitory signal transmission by the immunomodulatory receptor CD300a."53-0259 was used in Flow cytometry/Cell sorting to further define the mechanism by which CD300a acts as an inhibitor of immune cell receptor signalling, showing that CD300a is required for for inhibitory signal transmission."AuthorsDeBell KE,Simhadri VR,Mariano JL,Borrego F

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