CD25 Monoclonal Antibody (BC96), Biotin, eBioscience™

货号：13-0259-80,13-0259-82

规格：25 µg,100 µg

价格：0,1895

产品类型：流式抗体

品牌：eBioscience

抗原：CD25

物种：人/小鼠

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：BC96

荧光染料：Biotin

类型:	一抗	同型对照：	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Biotin, eBioscience™
浓度：	0.5 mg/mL	用法：	0.25 µg/test（Flow）

产品详细信息Description: The BC96 monoclonal antibody reacts with human CD25, the 55 kDa interleukin-2 receptor alpha chain (IL-2Ralpha). CD25 is expressed by early progenitors of T and B lineage as well as by activated mature T and B lymphocytes. By itself, CD25 binds IL-2 only with low affinity. However, CD25 associates with CD122 (IL-2 receptor beta chain) and CD132 (common gamma chain) to form the high affinity IL-2 receptor. CD25 plays a role in lymphocyte differentiating and activation/proliferation.<br>Applications Reported: This BC96 antibody has been reported for use in flow cytometric analysis.Applications Tested: This BC96 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD25 (IL2 receptor alpha chain/IL2RA) is a cytokine that plays a role in the proliferation of T and B lymphocytes. The receptor of this cytokine (IL2RA) is a heterotrimeric protein complex with a gamma chain also shared by interleukin 4 (IL4) and interleukin 7 (IL7). IL2RA, IL2R beta chain (IL2RB), and the IL2R gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric IL2RA chains result in low-affinity receptor, while homodimeric IL2RB chains produce a medium-affinity receptor. The expression of IL2 in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. IL2 is primarily produced by mature T cells. IL2 plays an important role as a growth factor, differentiation factor, and regulator of cell death. IL-2 stimulates the proliferation of B cells, augments natural killer cell activity, and inhibits granulocyte macrophage colony formation. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests a role in the immune response to antigenic stimuli. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.仅用于科研。不用于诊断过程。

数据

CD25 Antibody (13-0259-80)Investigative ophthalmology and visual science 2019 -Figure 3 T-lymphocyte subset distribution in glaucoma. Mononuclear cells isolated from peripheral blood samples from glaucoma (n = 32) and control (n = 21) groups were analyzed by multicolor flow cytometry for T-cell subset markers. (A) Similar to CD4+ Tregs, the percentage of CD8+/CD25+/FoxP3+ Tregs (CD8-Tregs) was also significantly lower in glaucomatous samples than nonglaucomatous controls (ANOVA, P < 0.001). (B) Shown are representative flow cytometry images after CD8/CD25/FoxP3 immunostaining for Tregs.

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参考文献

1.Acta Cardiologica SinicaThe Effects of High Mobility Group Box-1 Protein on Peripheral Treg/Th17 Balance in Patients with Atherosclerosis."Published figure using CD25 monoclonal antibody (Product # 13-0259-82) in Flow Cytometry"2.Scientific reportsCombinations of isoform-targeted histone deacetylase inhibitors and bryostatin analogues display remarkable potency to activate latent HIV without global T-cell activation."Published figure using CD25 monoclonal antibody (Product # 13-0259-82) in Flow Cytometry"3.Experimental and therapeutic medicineEquilibrium of Treg/Th17 cells of peripheral blood in syphilitic patients with sero-resistance."Published figure using CD25 monoclonal antibody (Product # 13-0259-82) in Flow Cytometry"4.International immunologyIL-1β and TNFα-initiated IL-6-STAT3 pathway is critical in mediating inflammatory cytokines and RANKL expression in inflammatory arthritis."13-0259 was used in Functional assays to elucidate the role of cytokines in chronic inflammation and joint destruction in rheumatoid arthritis."AuthorsMori T,Miyamoto T,Yoshida H,Asakawa M,Kawasumi M,Kobayashi T,Morioka H,Chiba K,Toyama Y,Yoshimura A5.Cell death and diseaseHSCARG downregulates NF-κB signaling by interacting with USP7 and inhibiting NEMO ubiquitination."13-0259 was used in Magnetic cell separation to investigate the effect of HSCARG on NF-κB signalling."AuthorsLi T,Guan J,Li S,Zhang X,Zheng X

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