CD25 Monoclonal Antibody (BC96), PE-Cyanine7, eBioscience™

货号：25-0259-42,25-0259-41

规格：100Tests,25Tests

价格：3035,1518

产品类型：流式抗体

品牌：eBioscience

抗原：CD25

物种：人

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：BC96

荧光染料：PE-Cy7

类型:	一抗	同型对照：	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE-Cyanine7, eBioscience™
浓度：	5 µL/Test	用法：	5 µL (0.25 µg)/test（Flow）

产品详细信息Description: The BC96 monoclonal antibody reacts with human CD25, the 55 kDa interleukin-2 receptor alpha chain (IL-2Ralpha). CD25 is expressed by early progenitors of T and B lineage as well as by activated mature T and B lymphocytes. By itself, CD25 binds IL-2 only with low affinity. However, CD25 associates with CD122 (IL-2 receptor beta chain) and CD132 (common gamma chain) to form the high affinity IL-2 receptor. CD25 plays a role in lymphocyte differentiating and activation/proliferation.<br>Applications Reported: The BC96 antibody has been reported for use in flow cytometric analysis.Applications Tested: This BC96 antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral blood. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD25 (IL2 receptor alpha chain/IL2RA) is a cytokine that plays a role in the proliferation of T and B lymphocytes. The receptor of this cytokine (IL2RA) is a heterotrimeric protein complex with a gamma chain also shared by interleukin 4 (IL4) and interleukin 7 (IL7). IL2RA, IL2R beta chain (IL2RB), and the IL2R gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric IL2RA chains result in low-affinity receptor, while homodimeric IL2RB chains produce a medium-affinity receptor. The expression of IL2 in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. IL2 is primarily produced by mature T cells. IL2 plays an important role as a growth factor, differentiation factor, and regulator of cell death. IL-2 stimulates the proliferation of B cells, augments natural killer cell activity, and inhibits granulocyte macrophage colony formation. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests a role in the immune response to antigenic stimuli. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.仅用于科研。不用于诊断过程。

数据

CD25 Antibody (25-0259-42) in FlowStaining of normal human peripheral blood cells with Anti-Human CD4 PE (Product # 12-0049-42) and Mouse IgG1 kappa Isotype Control PE-Cyanine7 (Product # 25-4714-80) (left) or Anti-Human CD25 PE-Cyanine7 (right). Cells in the lymphocyte gate were used for analysis. CD25 Antibody (25-0259-42)Experimental and therapeutic medicine 2016 -Figure 1. Flow cytometric mapping of the CD4 + CD25 + Foxp3 + cells in peripheral blood withdrawn from the syphilitic patients with sero-resistance. Foxp3, forkhead box P3.

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参考文献

1.BloodElevated frequencies of highly activated CD4+ T cells in HIV+ patients developing immune reconstitution inflammatory syndrome."25-0259 was used in Flow cytometry/Cell sorting to indicate that IRIS appears to be a predominantly CD4-mediated phenomenon."AuthorsAntonelli LR,Mahnke Y,Hodge JN,Porter BO,Barber DL,DerSimonian R,Greenwald JH,Roby G,Mican J,Sher A,Roederer M,Sereti I2.Journal of immunology (Baltimore, Md. : 1950)Human Head and Neck Squamous Cell Carcinoma-Associated Semaphorin 4D Induces Expansion of Myeloid-Derived Suppressor Cells."Published figure using CD25 monoclonal antibody (Product # 25-0259-42) in Flow Cytometry"3.ImmunologyImmune cellular parameters of leprosy and human immunodeficiency virus-1 co-infected subjects."25-0259 was used in Flow cytometry/Cell sorting to explore several aspects of cellular immunity in patients co-infected with human immunodeficiency virus-1 and Mycobacterium leprae."AuthorsCarvalho KI,Maeda S,Marti L,Yamashita J,Haslett PA,Kallas EG4.TransplantationRhesus monkey immature monocyte-derived dendritic cells generate alloantigen-specific regulatory T cells from circulating CD4+CD127-/lo T cells."25-0259 was used in Flow cytometry/Cell sorting to exhibit a novel method for the generation of non-human primate regulatory T cells with alloantigen specificity."AuthorsZahorchak AF,Raimondi G,Thomson AW5.ImmunologyImmune cellular parameters of leprosy and human immunodeficiency virus-1 co-infected subjects."25-0259 was used in Flow cytometry/Cell sorting to explore several aspects of cellular immunity in patients co-infected with human immunodeficiency virus-1 and Mycobacterium leprae."AuthorsCarvalho KI,Maeda S,Marti L,Yamashita J,Haslett PA,Kallas EG

技术参数

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