CD25 Monoclonal Antibody (BC96), PerCP-Cyanine5.5, eBioscience™

货号：45-0259-42

规格：100 Tests

价格：3419

产品类型：流式抗体

品牌：eBioscience

抗原：CD25

物种：人

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：BC96

荧光染料：PerCP-Cy5.5

类型:	一抗	同型对照：	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PerCP-Cyanine5.5, eBioscience™
浓度：	5 µL/Test	用法：	5 µL (0.125 µg)/test（Flow）

产品详细信息Description: The BC96 monoclonal antibody reacts with human CD25, the 55 kDa interleukin-2 receptor alpha chain (IL-2Ralpha). CD25 is expressed by early progenitors of T and B lineage as well as by activated mature T and B lymphocytes. By itself, CD25 binds IL-2 only with low affinity. However, CD25 associates with CD122 (IL-2 receptor \beta chain) and CD132 (common gamma chain) to form the high affinity IL-2 receptor. CD25 plays a role in lymphocyte differentiating and activation/proliferation.Applications Reported: This BC96 antibody has been reported for use in flow cytometric analysis.Applications Tested: This BC96 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488 nm; Emission: 695 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD25 (IL2 receptor alpha chain/IL2RA) is a cytokine that plays a role in the proliferation of T and B lymphocytes. The receptor of this cytokine (IL2RA) is a heterotrimeric protein complex with a gamma chain also shared by interleukin 4 (IL4) and interleukin 7 (IL7). IL2RA, IL2R beta chain (IL2RB), and the IL2R gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric IL2RA chains result in low-affinity receptor, while homodimeric IL2RB chains produce a medium-affinity receptor. The expression of IL2 in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. IL2 is primarily produced by mature T cells. IL2 plays an important role as a growth factor, differentiation factor, and regulator of cell death. IL-2 stimulates the proliferation of B cells, augments natural killer cell activity, and inhibits granulocyte macrophage colony formation. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests a role in the immune response to antigenic stimuli. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.仅用于科研。不用于诊断过程。

数据

CD25 Antibody (45-0259-42) in FlowStaining of normal human peripheral blood cells with Anti-Human CD4 APC (Product # 17-0049-42) and staining buffer (autofluorescence) (left) or Anti-Human CD25 PerCP-Cyanine5-5 (right). Cells in the lymphocyte gate were used for analysis.

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参考文献

1. Journal of immunology (Baltimore, Md. : 1950)Human Head and Neck Squamous Cell Carcinoma-Associated Semaphorin 4D Induces Expansion of Myeloid-Derived Suppressor Cells."Published figure using CD25 monoclonal antibody (Product # 45-0259-42) in Flow Cytometry"2. Experimental and therapeutic medicineEquilibrium of Treg/Th17 cells of peripheral blood in syphilitic patients with sero-resistance."Published figure using CD25 monoclonal antibody (Product # 45-0259-42) in Flow Cytometry"3. Journal of immunology (Baltimore, Md. : 1950)IL-15 Promotes Polyfunctional NK Cell Responses to Influenza by Boosting IL-12 Production.AuthorsWagstaffe HR,Nielsen CM,Riley EM,Goodier MR4. RetrovirologyHIV-1 latency and virus production from unintegrated genomes following direct infection of resting CD4 T cells."45-0259 was used in Flow cytometry/Cell sorting to demonstrate that unintegrated genomes of HIV-1 contribute to the overall effect of infection of resting CD4 T cells."AuthorsChan CN,Trinité B,Lee CS,Mahajan S,Anand A,Wodarz D,Sabbaj S,Bansal A,Goepfert PA,Levy DN5. OncoimmunologyA phase I dose-escalation clinical trial of a peptide-based human papillomavirus therapeutic vaccine withCandidaskin test reagent as a novel vaccine adjuvant for treating women with biopsy-proven cervical intraepithelial neoplasia 2/3."45-0259 was used in Flow cytometry/Cell sorting to demonstrate the safety of a human papillomavirus therapeutic vaccine, PepCan."AuthorsGreenfield WW,Stratton SL,Myrick RS,Vaughn R,Donnalley LM,Coleman HN,Mercado M,Moerman-Herzog AM,Spencer HJ,Andrews-Collins NR,Hitt WC,Low GM,Manning NA,McKelvey SS,Smith D,Smith MV,Phillips AM,Quick CM,Jeffus SK,Hutchins LF,Nakagawa M

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