CD4 Monoclonal Antibody (RPA-T4), PE-Cyanine7
货号：25-0049-41,25-0049-42
规格：25 Tests,100 Tests
价格：1416,2794
产品类型：流式抗体
品牌：Thermo Fisher
物种：人
宿主：小鼠
抗体亚型：IgG1, kappa
荧光染料：PE-Cy7
| 抗体类型 | 单抗 | 同型对照： | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE-Cyanine7 |
| 浓度： | 5 µL/Test | 用法： | 5 μL (0.5 μg)/test (Flow) |
| 产品详细信息Description: The RPA-T4 monoclonal antibody reacts with human CD4, a 59 kDa cell surface receptor expressed by a majority of thymocytes, subpopulation of mature T cells (T-helper cells) and in low levels on monocytes. CD4 is a receptor for the human immunodeficiency virus (HIV). RPA-T4 blocks HIV binding and mixed lymphocyte reaction. The RPA-T4 antibody recognizes a different epitope than the OKT4 monoclonal antibody, and these antibodies do not cross-block binding to each other's respective epitopes.Applications Reported: The RPA-T4 antibody has been reported for use in flow cytometric analysis.Applications Tested: This RPA-T4 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 4 µL (1 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息The CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV. CD4 is primarily expressed in a subset of T-lymphocytes, also referred to as T helper cells, but may also be expressed by other cells in the immune system, such as monocytes, macrophages, and dendritic cells. At the tissue level, CD4 expression may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues. CD4 functions to initiate or augment the early phase of T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase, Lck. It may also function as an important mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcripts have been identified in this gene [RefSeq, July 2017]. |
| 数据 |
| CD4 Antibody (25-0049-41) in FlowStaining of normal human peripheral blood cells with staining buffer (autofluorescence) (open histogram) or Anti-Human CD4 PE-Cyanine7 (filled histogram). Cells in the lymphocyte gate were used for analysis. |
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| 参考文献： |
| 1.Molecular pharmaceuticsModulation of CD4+ T lymphocyte lineage outcomes with targeted, nanoparticle-mediated cytokine delivery."Published figure using CD4 monoclonal antibody (Product # 25-0049-42) in Flow Cytometry"2.Acta Cardiologica SinicaThe Effects of High Mobility Group Box-1 Protein on Peripheral Treg/Th17 Balance in Patients with Atherosclerosis."25-0049 was used in Flow cytometry/Cell sorting to explore the mechanism of regulatory T cell/Th17 imbalance and the role of high mobility group box-1 protein on the balance in atherosclerosis."3.PloS oneIncreased frequencies of Th22 cells as well as Th17 cells in the peripheral blood of patients with ankylosing spondylitis and rheumatoid arthritis."Published figure using CD4 monoclonal antibody (Product # 25-0049-42) in Flow Cytometry"4.Scientific reportsTwo phase kinetics of the inflammatory response from hepatocyte-peripheral blood mononuclear cell interactions."25-0049 was used in Flow cytometry/Cell sorting to study the role of interactions between non and activated human peripheral blood mononuclear cells and human hepatoma HepaRG cells in the inflammatory response."AuthorsBeringer A,Molle J,Bartosch B,Miossec P5.CellRAB11FIP5 Expression and Altered Natural Killer Cell Function Are Associated with Induction of HIV Broadly Neutralizing Antibody Responses."Published figure using CD4 monoclonal antibody (Product # 25-0049-42) in Flow Cytometry" |
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