CD28 Monoclonal Antibody (CD28.2), Biotin

货号：13-0289-82

规格：100 ug

价格：1822

产品类型：流式抗体

品牌：eBioscience

抗原：CD28

物种：人

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：CD28.2

荧光染料：Biotin

类型:	单抗	同型对照：	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Biotin
浓度：	0.5 mg/mL	用法：	1 µg/test(Flow)

产品详细信息Description: The CD28.2 monoclonal antibody reacts with the human CD28 molecule, a 44 kDa homodimer expressed by thymocytes, mature T cells and plasma cells. CD28 is a ligand for CD80 (B7-1) and CD86 (B7-2) and is a potent co-stimulator of T cells. Signaling through CD28 augments IL-2 and IL-2 receptor expression as well as cytotoxicity of CD3-activated T cells.Applications Reported: The CD28.2 antibody has been reported for use in flow cytometric analysis.Applications Tested: The CD28.2 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD28 antigen is a 44 kDa disulfide linked homodimeric T cell specific surface glycoprotein. CD28 is a cell adhesion molecule of the immunoglobulin superfamily which is constitutively expressed on most peripheral blood T lymphocytes. Moreover, CD28 is the critical T cell costimulatory receptor that provides the cell the important second activation signal by binding CD80 and CD86 which are expressed by antigen presenting cells. In addition to its co-stimulation role, CD28 functions by preventing Tcells from entering an anergic-hyporesponsive state or from undergoing premature apoptotic cell death. In murine peripheral lymphoid organs and in the blood, all CD4+ and CD8+ T cells express CD28. In the thymus, CD28 expression is highest on immature CD3-, CD8+ and CD4+8+ cells, and on CD4-8- cells that express alpha/beta and gamma/delta TCR. The level of CD28 on mature CD4+ and CD8+ alpha/beta TCR+ thymocytes is two- to fourfold lower than on the immature cells. Diseases associated with CD28 dysfunction include mycosis fungiodes and Sezary's Disease.

数据

CD28 Antibody (13-0289-82) in FlowStaining of normal human peripheral blood cells with Anti-Human CD28 Biotin followed by detection with Streptavidin PE (Product # 12-4317-87). Cells in the lymphocyte gate were used for analysis. CD28 Antibody (13-0289-82)Molecular medicine reports 2016 -Published figure using CD28 monoclonal antibody (Product # 13-0289-82)

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参考文献

1. AutophagyA novel method for autophagy detection in primary cells: impaired levels of macroautophagy in immunosenescent T cells.2. Proceedings of the National Academy of Sciences of the United States of AmericaDramatic increase in naive T cell turnover is linked to loss of naive T cells from old primates.3. PloS oneFunctional characterization of T-cells from palatine tonsils in patients with chronic tonsillitis.4. Cell communication and signaling : CCSTCR activation kinetics and feedback regulation in primary human T cells.5. Journal of immunology (Baltimore, Md. : 1950)T cell-signaling network analysis reveals distinct differences between CD28 and CD2 costimulation responses in various subsets and in the MAPK pathway between resting and activated regulatory T cells.

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