CD28 Monoclonal Antibody (CD28.2), PE-eFluor 610

货号：61-0289-41,61-0289-42

规格：25 T,100 T

价格：1215,3035

产品类型：流式抗体

品牌：eBioscience

抗原：CD28

物种：人

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：CD28.2

荧光染料：PE-eFluor 610

类型:	单抗	同型对照：	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE-eFluor 610
浓度：	5 µL/Test	用法：	5 µL (0.25 µg)/test(Flow)

产品详细信息Description: The CD28.2 monoclonal antibody reacts with the human CD28 molecule, a 44 kDa homodimer expressed by thymocytes, mature T cells and plasma cells. CD28 is a ligand for CD80 (B7-1) and CD86 (B7-2) and is a potent co-stimulator of T cells. Signaling through CD28 augments IL-2 and IL-2 receptor expression as well as cytotoxicity of CD3-activated T cells.Applications Reported: This CD28.2 antibody has been reported for use in flow cytometric analysis.Applications Tested:This CD28.2 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.PE-eFluor® 610 can be excited with laser lines from 488-561 nm and emits at 607 nm. We recommend using a 610/20 band pass filter (equivalent to PE-Texas Red®). Please make sure that your instrument is capable of detecting this fluorochome.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 607 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD28 antigen is a 44 kDa disulfide linked homodimeric T cell specific surface glycoprotein. CD28 is a cell adhesion molecule of the immunoglobulin superfamily which is constitutively expressed on most peripheral blood T lymphocytes. Moreover, CD28 is the critical T cell costimulatory receptor that provides the cell the important second activation signal by binding CD80 and CD86 which are expressed by antigen presenting cells. In addition to its co-stimulation role, CD28 functions by preventing Tcells from entering an anergic-hyporesponsive state or from undergoing premature apoptotic cell death. In murine peripheral lymphoid organs and in the blood, all CD4+ and CD8+ T cells express CD28. In the thymus, CD28 expression is highest on immature CD3-, CD8+ and CD4+8+ cells, and on CD4-8- cells that express alpha/beta and gamma/delta TCR. The level of CD28 on mature CD4+ and CD8+ alpha/beta TCR+ thymocytes is two- to fourfold lower than on the immature cells. Diseases associated with CD28 dysfunction include mycosis fungiodes and Sezary's Disease.

数据

CD28 Antibody (61-0289-41) in FlowStaining of normal human peripheral blood cells with Anti-Human CD19 APC (Product # 17-0199-42) and Mouse IgG1 K Isotype Control PE-eFluor® 610 (Product # 61-4714-82) (left) or Anti-Human CD28 PE-eFluor® 610 (right). Cells in the lymphocyte gate were used for analysis. CD28 Antibody (61-0289-41)Molecular medicine reports 2016 -Published figure using CD28 monoclonal antibody (Product # 61-0289-41)

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参考文献

1. Journal for immunotherapy of cancerDetailed characterization of tumor infiltrating lymphocytes in two distinct human solid malignancies show phenotypic similarities.2. AutophagyA novel method for autophagy detection in primary cells: impaired levels of macroautophagy in immunosenescent T cells.3. Molecular medicine reportsCD28 family of receptors on Tcells in chronic HBV infection: Expression characteristics, clinical significance and correlations with PD-1 blockade.4. AutophagyA novel method for autophagy detection in primary cells: impaired levels of macroautophagy in immunosenescent T cells.

技术参数

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