FOXP3 Monoclonal Antibody (PCH101), FITC, eBioscience™

货号：11-4776-42,11-4776-41

规格：100Tests,25Tests

价格：3478,1954

产品类型：流式抗体

品牌：eBioscience

抗原：FOXP3

物种：人

宿主：大鼠

抗体亚型：IgG2a, kappa

克隆号：PCH101

荧光染料：FITC

类型:	一抗	同型对照：	Rat IgG2a kappa Isotype Control (eBR2a), FITC, eBioscience™
浓度：	5 µL/Test	用法：	5 µL (0.5 µg)/test

产品详细信息Description: eBioscience offers a panel of monoclonal antibodies to different epitopes of human Foxp3, providing useful tools for investigating the complete expression pattern of Foxp3 at the protein level, and discerning the precise subsets of Foxp3^+ cells. Please contact tech@ebioscience.com or 888.810.6168 for any additional assistance.The PCH101 antibody reacts with the amino terminus of human foxp3 protein also known as FORKHEAD BOX P3, SCURFIN, and JM2; cross reactivity of this antibody to other proteins has not been determined. Foxp3, a 49-55 kDa protein, is a member of the forkhead/winged-helix family of transcriptional regulators, and was identified as the gene defective in 'scurfy' (sf) mice. Constitutive high expression of Foxp3 mRNA has been shown in CD4+CD25+ regulatory T cells (Treg cells), and ectopic expression of foxp3 in CD4+CD25- cells imparts a Treg phenotype in these cells.Intracellular staining of human peripheral blood mononuclear cells (PBMCs) with PCH101 antibody using the anti-human Foxp3 Staining Set and protocol reveals approximately 0.5-4% of lymphocytes staining, with the majority of staining occurring in the CD25^bright population. This is subject to donor variability.PCH101 crossreacts with rhesus, chimpanzee and cynomolgus. We recommend the use of CD4 (OKT4, cat. 11-0048, or RPA-T4, cat. 11-0049, depending on the species) and CD25 (BC96, cat. 17-0259).Applications Reported: This PCH101 antibody has been reported for use in intracellular staining followed by flow cytometric analysis and immunohistology (both paraffin and frozen sections).Applications Tested: This PCH101 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of normal human peripheral blood cells using the Anti-Human Foxp3 FITC Staining Set (cat. 71-5776). This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息FOXP3 (Forkhead box protein 3) is a member of the forkhead/winged-helix family of transcriptional regulators, highly conserved across mammals, and essential for normal immune homeostasis. FOXP3 is 381 amino acids long, stably and constitutively expressed at a high level in CD25 + CD4 positive regulatory T cells, a low level in CD4-positive/CD25-negative cells, and is absent in CD4-negative/CD8-positive T cells. FOXP3 may be a master regulatory gene, and a more specific marker of regulatory T cells. Defects in the gene encoding FOXP3 protein cause the scurfy phenotype in mice. In humans FOXP3 defects play a role in IPEX syndrome (immune dysfunction, polyendocrinopathy, enteropathy, X-linked syndrome), also known as X-linked autoimmunity-allergic dysregulation (XLAAD) syndrome. Transcript variants of FOXP3 encoding different isoforms have been identified. In human breast and colon cancer cells, expression of FOXP3 is regulated by p53 in response to the DNA damage.仅用于科研。不用于诊断过程。

数据

FOXP3 Antibody (11-4776-41) in FlowSurface staining of normal human peripheral blood cells with Anti-Human CD4 PE (Product # 12-0049-42) (left) and Anti-Human CD25 APC (Product # 17-0259-42) (right) and subsequently fixed and permeabilized using the Foxp3 Staining Buffers (Product # 00-5523-00) and stained with Anti-Human Foxp3 FITC. Cells in the lymphocyte gate were used for analysis.

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参考文献

1.Journal of oncologySecretory High-Mobility Group Box 1 Protein Affects Regulatory T Cell Differentiation in Neuroblastoma MicroenvironmentIn Vitro."11-4776 was used in Flow cytometry/Cell sorting to elucidate the role of the neuroblastoma microenvironment in promoting regulatory T cell phenotype."AuthorsVanichapol T,Chiangjong W,Panachan J,Anurathapan U,Chutipongtanate S,Hongeng S2.International journal of nanomedicineFunctionalization-dependent effects of cellulose nanofibrils on tolerogenic mechanisms of human dendritic cells."11-4776 was used in Flow cytometry/Cell sorting to investigate how differentially functionalised cellulose nanofibrils affect dendritic cell properties, their viability, morphology, differentiation and maturation potential, and the capacity to regulate T cell-mediated immune response."AuthorsTomić S,Ilić N,Kokol V,Gruden-Movsesijan A,Mihajlović D,Bekić M,Sofronić-Milosavljević L,Čolić M,Vučević D3.Scientific reportsClinical significance of CCR7+CD8+T cells in kidney transplant recipients with allograft rejection."11-4776 was used in Flow cytometry/Cell sorting to reveal the impact of CCR7+CD8+ T cells has on the proliferation and differentiation of T cells into effector cells in kidney transplant patients."AuthorsKim KW,Kim BM,Doh KC,Cho ML,Yang CW,Chung BH4.BloodMucosal but not peripheral FOXP3+ regulatory T cells are highly increased in untreated HIV infection and normalize after suppressive HAART.AuthorsEpple HJ,Loddenkemper C,Kunkel D,Tröger H,Maul J,Moos V,Berg E,Ullrich R,Schulzke JD,Stein H,Duchmann R,Zeitz M,Schneider T5.Cancer immunology, immunotherapy : CIIVaccine-specific local T cell reactivity in immunotherapy-associated vitiligo in melanoma patients."11-4776 was used in Immunohistochemistry to show that functional CD8(+) T cells specific for melanoma differentiation antigens used in a melanoma immunotherapy trial, do not only invade the tumor, but also the vitiligo lesions."AuthorsJacobs JF,Aarntzen EH,Sibelt LA,Blokx WA,Boullart AC,Gerritsen MJ,Hoogerbrugge PM,Figdor CG,Adema GJ,Punt CJ,de Vries IJ

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