CD69 Monoclonal Antibody (FN50), PE-Cyanine7, eBioscience™

货号：25-0699-41,25-0699-42

规格：25tests,100tests

价格：1518,3035

产品类型：流式抗体

品牌：Thermo Fisher

物种：人

宿主：小鼠

抗体亚型：IgG1, kappa

荧光染料：PE-Cy7

抗体类型:	流式抗体	同型对照：	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE-Cyanine7, eBioscience
浓度：	5 µL/Test	用法：	5 µL (0.125 µg)/test (Flow）

产品详细信息Description:The FN50 monoclonal antibody reacts with human CD69, also known as very early activation antigen (VEA). CD69 is approximately 30 kDa and is expressed on the cell-surface as a disulfide-linked dimer. CD69 is rapidly upregulated upon activation and expressed on lymphocytes, monocytes and platelets.Applications Reported: This FN50 antibody has been reported for use in flow cytometric analysis.Applications Tested: This FN50 antibody has been pre-titrated and tested by flow cytometric analysis of resting and 6-hour TPA-activated human normal human peripheral blood cells. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.Target InformationCD69 (AIM, Active Inducer Molecule) is a gp28/34 disulfide bonded homodimer with a molecular weight of 60 kDa under non-reducing conditions. CD69 contains one or two N linked oligosacaride and the molecule is present on activated platelets. In normal peripheral blood a variable percentage of cells express the CD69 antigen, and it is involved in lymphocyte signal transduction. Expression CD69 is induced upon activation of T lymphocytes, and may play a role in proliferation. Furthermore, the protein may act to transmit signals in natural killer cells and platelets. Alternative splicing results in multiple transcript variants of CD69. Diseases associated with CD69 dysfunction include coccidiodomycosis and asthma.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

CD69 Antibody (25-0699-41) in FlowStaining of PHA-stimulated normal human peripheral blood cells with Mouse IgG1 K Isotype Control PE-Cyanine7 (Product # 25-4714-80) (open histogram) or Anti-Human CD69 PE-Cyanine7 (filled histogram). Total cells were used for analysis.

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参考文献：

1.eLifeHIV Tat controls RNA Polymerase II and the epigenetic landscape to transcriptionally reprogram target immune cells."Published figure using CD69 monoclonal antibody (Product # 25-0699-42) in Flow Cytometry"2.Nature communicationsRSV-specific airway resident memory CD8+ T cells and differential disease severity after experimental human infection."Published figure using CD69 monoclonal antibody (Product # 25-0699-42) in Flow Cytometry"3.hinese medicineGlossogyne tenuifolia(Hsiang-ju) extract suppresses T cell activation by inhibiting activation of c-Jun N-terminal kinase."Published figure using CD69 monoclonal antibody (Product # 25-0699-42) in Flow Cytometry"

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