CD152 (CTLA-4) Monoclonal Antibody (14D3), Biotin, eBioscience™
货号:13-1529-82
规格:100μg
价格:2788
产品类型:流式抗体
品牌:Thermo Fisher
抗原:CD152
物种:其它
宿主:小鼠
抗体亚型:IgG2a, kappa
克隆号:14D3
荧光染料:Biotin
抗体类型: | 流式抗体 | 同型对照: | Mouse IgG2a kappa Isotype Control (eBM2a), Biotin, eBioscience |
浓度: | 0.5 mg/mL | 用法: | 0.06 µg/test (Flow) |
产品详细信息Description:The 14D3 monoclonal antibody reacts with human CD152, also known as cytotoxic T lymphocyte antigen-4 (CTLA-4). CTLA-4, a protein with structural similarities to CD28, is expressed on activated T cells (activated B cells may also express CTLA-4) and binds the B7 family members, CD80 (B7-1) and CD86 (B7-2), with higher affinity than CD28 does. CTLA-4 and CD28 appear to deliver opposing signals to T cells: while CD28 is a potent costimulator, CTLA-4 restricts the progression of T cellsto an activated state by inhibiting IL-2 secretion and cellular proliferation. The cytoplasmic portion of CTLA-4 contains ER retention motifs, resulting in intracellular localization of a large proportion of newly synthesized CTLA-4 in response to TCR signaling.The 14D3 antibody also recognizes rhesus monkey and has inhibitor activity.Applications Reported: This 14D3 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This 14D3 antibody has been tested This 14D3 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of stimulated human peripheral blood cells using the Intracellular Fixation and Permeabilization Buffer Set (cat. 88-8824) and protocol. Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online. This can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Furthermore, due to the intracellular localization of a large portion of CTLA-4, for complete detection it may be necessary to assess intracellular expression, in addition to surface expression of CTLA-4.Filtration: 0.2 µm post-manufacturing filtered.Target InformationCTLA4 (Cytotoxic T-Lymphocyte Antigen 4) is a CD28-family receptor expressed on mainly CD4+ T cells. It binds the same ligands as CD28 (CD80 and CD86 on B cells and dendritic cells), but with higher affinity than CD28. However, in contrast to CD28 which enhances cell function when bound at the same time as the T cell receptor, CTLA4 inhbits the T cell and prevents it from functioning. Intracellular CTLA4 is also found in regulatory T cells and may be important to their function.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
数据 |
CD152 (CTLA-4) Antibody (13-1529-82) in FlowPHA-stimulated human peripheral blood cells were intracellularly stained with 0.03 µg of Mouse IgG2a K Isotype Control Biotin (Product # 13-4724-85) (open histogram) or 0.03 µg of Anti-Human CD152 (CTLA-4) Biotin (filled histogram) followed by Streptavidin PE (Product # 12-4317-87) using the Intracellular Fixation and Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Total viable cells were used for analysis. |
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参考文献: |
1.Scientific reportsLow Expression of miR-424-3p is Highly Correlated with Clinical Failure in Prostate Cancer."Published figure using CD152 (CTLA-4) monoclonal antibody (Product # 13-1529-82) in Immunohistochemistry"2.Journal of CancerCD103+tumor-infiltrating lymphocytes predict favorable prognosis in patients with esophageal squamous cell carcinoma."Published figure using CD152 (CTLA-4) monoclonal antibody (Product # 13-1529-82) in Immunocytochemistry"3.OncotargetHDAC inhibition potentiates immunotherapy in triple negative breast cancer."Published figure using CD152 (CTLA-4) monoclonal antibody (Product # 13-1529-82) in Flow Cytometry"4.Nature communicationsFollicular regulatory T cells impair follicular T helper cells in HIV and SIV infection."Published figure using CD152 (CTLA-4) monoclonal antibody (Product # 13-1529-82) in Flow Cytometry" |
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