CD137 (4-1BB) Monoclonal Antibody (4B4 (4B4-1)), PerCP-eFluor 710
货号：46-1379-41,46-1379-42
规格：25 Tests,100 Tests
价格：1363,3360
产品类型：流式抗体
品牌：Thermo Fisher
物种：人
宿主：小鼠
抗体亚型：IgG1, kappa
荧光染料：PerCP-eFluor™ 710
| 抗体类型 | 单抗 | 同型对照： | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PerCP-eFluor 710 |
| 浓度： | 5 µL/Test | 用法： | 5 μL (0.125 μg)/test (Flow) |
| 产品详细信息Description: This 4B4 (4B4-1) monoclonal antibody reacts with human CD137 (also known as 4-1BB or TNFRSF9), which is an inducible member of the TNFR family of costimulatory molecules expressed on T cells, natural killer cells, dendritic cells, granulocytes, and mast cells. Involved in recruiting TNFR-associated factors (TRAF) 1 and 2, CD137 signaling plays a role in T cell activation, maintaining the survival of activated and CD8 memory T cells, as well as suppressing myelopoiesis and dendriticcell development. Stimulation of this receptor has also been shown to promote expansion of CD4+CD25+ T regulatory cells i{ex vivoi}. The ligand for CD137, 4-1BBL, is found on activated macrophages, mature B cells, hematopoietic stem cells, and myeloid progenitor cells.Applications Reported: This 4B4 (4B4-1) antibody has been reported for use in flow cytometric analysis.Applications Tested: This 4B4 (4B4-1) antibody has been pre-titrated and tested by flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD137, also known as TNFRSF9 or 4-1BB, is an inducible costimulatory molecule expressed mainly on activated T cells. Its ligand, known as 4-1BBL, is expressed on activated macrophages, mature B cells, hematopoietic stem cells, and myeloid progenitor cells. CD137 signaling leads to maintaining the survival of activated T cells and CD8+ memory T cells, and clonal expansion of T cells, but also to suppressing myelopoiesis and dendritic cell development. Triggered CD137 induces a cytokine release profile regulating peripheral monocyte survival. Soluble forms of CD137 may provide negative control mechanism for some immune responses. |
| 数据 |
| CD137 (4-1BB) Antibody (46-1379-41) in FlowStaining of 3-day Con A-stimulated normal human peripheral blood cells with Anti-Human CD25 APC (Product # 17-0257-42) and Mouse IgG1 K Isotype Control PerCP-eFluor® 710 (Product # 46-4714-82) (left) or Anti-Human CD137 (4-1BB) PerCP-eFluor® 710 (right). Viable cells, as determined by Fixable Viability Dye eFluor® 450 (Product # 65-0863-14), in the lymphocyte gate were used for analysis. |
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| 参考文献： |
| 1.Oxidative medicine and cellular longevityAntroquinonol Exerts Immunosuppressive Effect on CD8+T Cell Proliferation and Activation to Resist Depigmentation Induced by H2O2."Published figure using CD137 (4-1BB) monoclonal antibody (Product # 46-1379-42) in Flow Cytometry" |
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