CD152 (CTLA-4) Monoclonal Antibody (eBio20A (20A, A3)), PE, eBioscience™

货号：12-1528-42

规格：100tests

价格：2677

产品类型：流式抗体

品牌：Thermo Fisher

抗原：CD152

物种：人

宿主：小鼠

抗体亚型：IgG2a, kappa

克隆号：eBio20A (20A, A3)

荧光染料：PE

抗体类型:	流式抗体	同型对照：	Mouse IgG2a kappa Isotype Control (eBM2a), PE, eBioscience
浓度：	5 µL/Test	用法：	5 µL (0.125 µg)/test (Flow）

产品详细信息Description:The eBio20A monoclonal antibody reacts with human CD152, also known as cytotoxic T lymphocyte antigen-4 (CTLA-4). CTLA-4, a protein with structural similarities to CD28, is expressed on activated T cells (activated B cells may also express CTLA-4) and binds the B7 family members, CD80 (B7-1) and CD86 (B7-2), with higher affinity than CD28 does. CTLA-4 and CD28 appear to deliver opposing signals to T cells: while CD28 is a potent costimulator, CTLA-4 restricts the progression of T cells to an activated state by inhibiting IL-2 secretion and cellular proliferation. The cytoplasmic portion of CTLA-4 contains ER retention motifs, resulting in intracellular localization of a large proportion of newly synthesized CTLA-4 in response to TCR signaling.Applications Reported: This eBio20A (20A, A3) antibody has been reported for use in flow cytometric analysis.Applications Tested: This eBio20A (20A, A3) antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of PHA stimulated normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.Target InformationCTLA4 (Cytotoxic T-Lymphocyte Antigen 4) is a CD28-family receptor expressed on mainly CD4+ T cells. It binds the same ligands as CD28 (CD80 and CD86 on B cells and dendritic cells), but with higher affinity than CD28. However, in contrast to CD28 which enhances cell function when bound at the same time as the T cell receptor, CTLA4 inhbits the T cell and prevents it from functioning. Intracellular CTLA4 is also found in regulatory T cells and may be important to their function.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

CD152 (CTLA-4) Antibody (12-1528-42) in FlowIntracellular staining of 3-day PHA-stimulated normal human peripheral blood cells with Anti-Human CD3 APC (Product # 17-0038-42) and Mouse IgG2a kappa Isotype Control PE (Product # 12-4724-81) (left) or Anti-Human CD152 (CTLA-4) PE (right). Total viable cells were used for analysis.

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参考文献：

1.Journal of immunology (Baltimore, Md. : 1950)De novo generation and enhanced suppression of human CD4+CD25+ regulatory T cells by retinoic acid."12-1528 was used in Flow cytometry/Cell sorting to investigate methods to convert T cells into Treg cells, showing that retinoic acid can be used for de novo generation of Tregs."

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