CD45RA Monoclonal Antibody (HI100), APC, eBioscience™

货号：17-0458-42

规格：100 Tests

价格：2820

产品类型：流式抗体

品牌：eBioscience

抗原：CD45RA

物种：人

宿主：小鼠

抗体亚型：IgG2b, kappa

克隆号：HI100

荧光染料：APC

类型:	一抗	同型对照：	Mouse IgG2b kappa Isotype Control (eBMG2b), APC, eBioscience™
浓度：	5 µL/Test	用法：	5 µL (0.008 µg)/test（Flow）

产品详细信息Description: The HI100 monoclonal antibody reacts with human CD45RA, a 220 kDa molecule expressed by subpopulations of CD4+ peripheral T lymphocytes, CD8+ peripheral T lymphocytes, and B cells. The CD45RA+ T cell populations are mainly naive/virgin allowing the use of HI100 mAb as a phenotypic marker to discriminate T cell subsets.Applications Reported: This HI100 antibody has been reported for use in flow cytometric analysis.Applications Tested: This HI100 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 20 µL (.008 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing and these isoforms differ in their extracellular domains, however, they share identical transmembrane and cytoplasmic domains. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45 is expressed on cells of the human hematopoietic lineage, with the exception of mature red cells and is not detected on differentiated cells of other tissues. It is likely that CD45 plays an important role in signal transduction, inhibition or upregulation of various immunological functions. Diseases associated with CD45 dysfunction include severe combined immunodeficiency and Hepatitis C.仅用于科研。不用于诊断过程。

数据

CD45RA Antibody (17-0458-42) in FlowStaining of normal human peripheral blood cells with Mouse IgG2b K Isotype Control APC (Product # 17-4732-81) (open histogram) or Anti-Human CD45RA APC (filled histogram). Cells in the lymphocyte gate were used for analysis.

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文献

1.Frontiers in immunologyIdentifying the Presence of Prostate Cancer in Individuals with PSA Levels <20 ng ml-1Using Computational Data Extraction Analysis of High Dimensional Peripheral Blood Flow Cytometric Phenotyping Data."17-0458 was used in Flow cytometry/Cell sorting to present an advanced computational data extraction approach which can identify the presence of prostate cancer in men with PSA levels 20 ng ml-1 on the basis of peripheral blood immune cell profiles."AuthorsCosma G,McArdle SE,Reeder S,Foulds GA,Hood S,Khan M,Pockley AG2.Molecular medicine reportsInterleukin‑33‑induced immune tolerance is associated with the imbalance of memory and naïve T‑lymphocyte subsets."17-0458 was used in Flow cytometry/Cell sorting to investigate the distribution of memory and naive T cells in hepatitis B virus-infected patients at different immune stages, and the effect of interleukin-33 on the regulation of the T-cell subsets."AuthorsShi X,Tang Y,Sun X,Liu Y,Sun Y,Sun M,Jiang Y,Li Y3.Journal of immunology (Baltimore, Md. : 1950)CpG and non-CpG oligodeoxynucleotides directly costimulate mouse and human CD4+ T cells through a TLR9- and MyD88-independent mechanism."17-0458 was used in Flow cytometry/Cell sorting to study the ability of oligodeoxynucleotides to activate and polarise T cells."AuthorsLandrigan A,Wong MT,Utz PJ4.Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow TransplantationLong-term immune reconstitution of naive and memory tcell pools after haploidentical hematopoietic stem cell transplantation."17-0458 was used in Flow cytometry/Cell sorting to show that long-term immune reconstitution was successfully achieved after haploidentical hematopoietic stem cell transplantation, a process that appears to have largely relied on de novo T cell production."AuthorsAzevedo RI,Soares MV,Albuquerque AS,Tendeiro R,Soares RS,Martins M,Ligeiro D,Victorino RM,Lacerda JF,Sousa AE5.Molecular medicine reportsInterleukin‑33‑induced immune tolerance is associated with the imbalance of memory and naïve T‑lymphocyte subsets."17-0458 was used in Flow cytometry/Cell sorting to investigate the distribution of memory and naive T cells in hepatitis B virus-infected patients at different immune stages, and the effect of interleukin-33 on the regulation of the T-cell subsets."AuthorsShi X,Tang Y,Sun X,Liu Y,Sun Y,Sun M,Jiang Y,Li Y

技术参数

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