CD154 (CD40 Ligand) Monoclonal Antibody (24-31), Super Bright 436, eBioscience™
货号：62-1548-42
规格：100tests
价格：3104
产品类型：流式抗体
品牌：Thermo Fisher
抗原：CD154
物种：其它
宿主：小鼠
抗体亚型：IgG1, kappa
克隆号：24-31
荧光染料：Super Bright 436
| 抗体类型: | 流式抗体 | 同型对照： | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Super Bright 436, eBioscience |
| 浓度： | 5 µL/Test | 用法： | 5 µL (0.5 µg)/test (Flow） |
产品详细信息Description:The 24-31 monoclonal antibody reacts with human CD154, a 39 kDa transmembrane glycoprotein also known as gp39 and CD40 ligand (CD40L). CD154 is a member of the TNF superfamily and is expressed transiently by activated T cells. Through its binding to CD40 on antigen presenting cells including B cells, monocytes/macrophages and dendritic cells, CD154 serves a crucial function in T-APC cognate interaction. CD154 interaction with CD40 transduces signals for T-dependent B-cell activationand induces B cell cycle entry. 24-31 cross-reacts with the cynomolgus monkey CD154.Applications Reported: This 24-31 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 24-31 antibody has been pre-diluted and tested by flow cytometric analysis of stimulated normal human peripheral blood cells. This may be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm. We recommend using a 450/50 bandpass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome.When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.Excitation: 405 nm; Emission: 436 nm; Laser: Violet LaserSuper Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.Target InformationCD40 Ligand (CD40-L), or CD154, is a membrane glycoprotein and differentiation antigen expressed on the surface of T cells. The CD40 Ligand stimulates B cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines. CD40 Ligand has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. It also co-stimulates proliferation of activated T cells and this is accompanied by the production of IFN-gamma, TNF-alpha, and IL-2.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
| 数据 |
| CD154 (CD40 Ligand) Antibody (62-1548-42) in FlowNormal human peripheral blood cells cells were unstimulated (left) or stimulated for 5 hours with the Cell Stimulation Cocktail (plus protein transport inhibitors) (Product # 00-4975) (right). Cells were then stained with CD8 Monoclonal Antibody, FITC (Product # 11-0088) and CD154 (CD40 Ligand) Monoclonal Antibody, Super Bright 436 (Product # 62-1548-42). Cells in the lymphocyte gate were used for analysis. |
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