CD279 (PD-1) Monoclonal Antibody (MIH4), APC
货号：17-9969-41,17-9969-42
规格：25 Tests,100 Tests
价格：1577,3003
产品类型：流式抗体
品牌：Thermo Fisher
物种：人
宿主：小鼠
抗体亚型：IgG1, kappa
荧光染料：APC
| 抗体类型 | 单抗 | 同型对照： | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), APC |
| 浓度： | 5 µL/Test | 用法： | 5 μL (1 μg)/test (Flow) |
| 产品详细信息Description: The MIH4 monoclonal antibody reacts with the human PD-1 (programmed death-1), a 55 kDa member of the immunoglobulin superfamily. PD-1 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) and plays a key role in peripheral tolerance and autoimmune disease. PD-1 is expressed predominantly on activated T and B lymphocytes. Two novel members of the B7 family have been identified as the PD-1 ligands, PD-L1 (B7-H1) and PD-L2 (B7-DC). Evidence reported to date suggests overlapping functions for these two PD-1 ligands and their constitutive expression on some normal tissues and upregulation on activated antigen-presenting cells. The MIH4 antibody recognizes a different epitope than antibody clones J105.Applications Reported: This MIH4 antibody has been reported for use in flow cytometric analysis.Applications Tested: This MIH4 antibody has been pre-titrated and tested by flow cytometric analysis of PHA-stimulated normal human peripheral blood cells. This can be used at 5 µL (1 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antig en-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2. Upon binding to either of these ligands, signals generated by PD-1 inhibit the activation of theimmune response in the absence of "danger signals" such as LPS or other molecules associated with bacteria or other pathogens. Evidence for this is seen in PD1-null mice who exhibit hyperactivated immune systems and autoimmune diseases. Despite its predicted molecular weight, PD-1 often migrates at higher molecular weight in SDS-PAGE. |
| 数据 |
| CD279 (PD-1) Antibody (17-9969-41) in FlowStaining of untimulated (left) or 3-day PHA-stimulated (right) normal human peripheral blood cells with Mouse IgG1 K Isotype Control APC (Product # 17-4714-81) (blue histogram) or Anti-Human CD279 (PD-1) APC (purple histogram). Total viable cells were used for analysis. |
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| 参考文献： |
| 1.Nature communicationsImmunological biomarkers predict HIV-1 viral rebound after treatment interruption."17-9969 was used in Flow cytometry/Cell sorting to show that immunological biomarkers can predict time to viral rebound after stopping antiretroviral therapy."2.NatureT-cell exhaustion, co-stimulation and clinical outcome in autoimmunity and infection."17-9969 was used in Flow cytometry/Cell sorting to conclude that T-cell exhaustion plays a central role in determining outcome in autoimmune disease and targeted manipulation of this process could lead to new therapeutic opportunities."3.Journal for immunotherapy of cancerPD-1 signaling affects cristae morphology and leads to mitochondrial dysfunction in human CD8+T lymphocytes."17-9969 was used in Flow cytometry/Cell sorting to suggest that mitochondria are the main targets of PD-1 inhibitory activity. PD-1 reprograms CD8+ T cell metabolism for efficient use of fatty acid oxidation; this mitochondrial phenotype might explain the long-lived phenotype of PD-1-engaged T cells."4.Journal for immunotherapy of cancerPD-1 signaling affects cristae morphology and leads to mitochondrial dysfunction in human CD8+T lymphocytes."17-9969 was used in Flow cytometry/Cell sorting to suggest that mitochondria are the main targets of PD-1 inhibitory activity. PD-1 reprograms CD8+ T cell metabolism for efficient use of fatty acid oxidation; this mitochondrial phenotype might explain the long-lived phenotype of PD-1-engaged T cells."5.Cell reportsAntigen-Presenting Cell-Intrinsic PD-1 Neutralizes PD-L1 in cis to Attenuate PD-1 Signaling in T Cells."Published figure using CD279 (PD-1) monoclonal antibody (Product # 17-9969-42) in Flow Cytometry" |
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