CD45RO Monoclonal Antibody (UCHL1), PE-Cyanine5

货号：15-0457-42

规格：100 T

价格：2629

产品类型：流式抗体

品牌：eBioscience

抗原：CD45RO

物种：人

宿主：小鼠

抗体亚型：IgG2a, kappa

克隆号：UCHL1

荧光染料：PE-Cy5

类型:	单抗	同型对照：	Mouse IgG2a kappa Isotype Control (eBM2a), PE-Cyanine5
浓度：	5 µL/Test	用法：	5 µL (0.03 µg)/test(Flow)

产品详细信息Description: The UCHL1 monoclonal antibody reacts with human CD45RO, a 180 kDa isoform of CD45. CD45RO is expressed by most thymocytes, activated memory T cells, granulocytes and monocytes. CD22 is a ligand for CD45RO. Expression of CD45RO and CD45RA is used commonly to discriminate subsets of peripheral T cells.Applications Reported: The UCHL1 antibody has been reported for use in flow cytometric analysis.Applications Tested: This UCHL1 antibody has been pre-titrated and testedby flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.03 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 667 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD45RO (UCHL1, ubiquitin carboxyl terminal esterase L1) is the shortest isoform of a receptor-type protein tyrosine phosphatase, CD45 glycoprotein. CD45 is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases, promotes cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45 isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45RO is expressed on macrophages, CD8+ T cells, activated T cells and myeloma cells. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis.

数据

CD45RO Antibody (15-0457-42) in FlowStaining of normal human peripheral blood cells with staining buffer (autofluorescence) (open histogram) or Anti-Human CD45RO PE-Cyanine5 (filled histogram).Cells in the lymphocyte gate were used for analysis.

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参考文献

1. OncoimmunologyFunctional expression of CD137 (4-1BB) on T helper follicular cells.2. Cell researchBlocking the recruitment of naive CD4+T cells reverses immunosuppression in breast cancer.3. JCI insightSingle-cell profiling reveals GPCR heterogeneity and functional patterning during neuroinflammation.

技术参数

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