Rat IgG2a kappa Isotype Control (eBR2a)

货号：14-4321-81,14-4321-82,14-4321-85

规格：50 ug,100 ug,500 ug

价格：721,967,1940

产品类型：大鼠同型对照

品牌：eBioscience

物种：其它

宿主：大鼠

抗体亚型：IgG2a, kappa

克隆号：eBR2a

荧光染料：其它

类型:	同型对照	同型对照：
浓度：	0.5 mg/mL	用法：	Assay-Dependent(Flow)；Assay-Dependent(IHC)；Assay-Dependent(Ctrl)；Assay-Dependent(ICC)；Assay-Dependent(IF)

产品详细信息Description: The monoclonal rat IgG2a, kappa is useful as an isotype control immunoglobulin.Applications Reported: This rat IgG2a isotype control has been reported for use in immunocytochemistry, immunohistochemistry, flow cytometric analysis, and immunoglobulin ELISA.Applications Tested: This rat IgG2a isotype control has been used by flow cytometric analysis of mouse splenocyte suspension and should be used at the same concentration as the experimental antibody.Purity:Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2 µm post-manufacturing filtered.靶标信息The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.

数据

Rat IgG2a kappa Isotype Control (14-4321-81) in IHC (P)Immunohistochemistry of formalin-fixed paraffin embedded human tonsil tissue using 5 µg/mL Rat IgG2a K Isotype Control Purified (left) or 5 µg/mL Anti-Human Human Granzyme B Purified (right) followed by Anti-Rat IgG Biotin and DAB visulization.Nuclei are stained with hematoxylin.

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参考文献

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