Mouse IgG2b kappa Isotype Control (eBMG2b)
货号：14-4732-81,14-4732-82,14-4732-85
规格：50μg,100μg,500μg
价格：582,1044,1283
产品类型：小鼠同型对照
品牌：eBioscience
物种：其它
宿主：小鼠
抗体亚型：IgG2b, kappa
荧光染料：其它
| 抗体类型 | 单抗 | 同型对照： | |
| 浓度： | 0.5 mg/mL | 用法： | Assay-Dependent (Flow)；Assay-Dependent (Ctrl) |
| 产品详细信息Description: The monoclonal mouse IgG2b, kappa is useful as an isotype control immunoglobulin.Applications Reported: This mouse IgG2b isotype control has been reported for use in surface and intracellular flow cytometric analysis, immunocytochemistry, immunohistochemistry.Applications Tested: This mouse IgG2b Isotype Control has been tested by flow cytometric analysis of normal human peripheral blood cells. Use isotype control at the same concentration as experimental antibody.<br>Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2 µm post-manufacturing filtered.靶标信息The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody. |
| 数据 |
| Mouse IgG2b kappa Isotype Control (14-4732-81) in IHC (P)Immunohistochemistry of formalin-fixed paraffin embedded human skin tissue using 0.1 µg/mL of Mouse IgG2b K Isotype Control Purified (left) or 0.1 µg/mL of Anti-Human IL-38 Purified (right) followed by Anti-Mouse Biotin, Streptavidin-HRP, and DAB visualization.Nuclei are counterstained with hematoxylin. |
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| 参考文献： |
| 1.TheranosticsClinical efficacy of omalizumab in chronic spontaneous urticaria is associated with a reduction of FcεRI-positive cells in the skin."14-4732 was used in Immunohistochemistry to discover that treatment with omalizumab was associated with reduction in FcɛRI+and IgE+basophils and intradermal cells."2.Journal of virologyHerpes simplex virus 1 glycoprotein B and US3 collaborate to inhibit CD1d antigen presentation and NKT cell function."14-4732 was used in Western blotting following immunoprecipitation to study the mechanisms utilised by Herpes simplex virus 1 to inhibit natural killer T cell function during the initial antiviral response."AuthorsRao P,Pham HT,Kulkarni A,Yang Y,Liu X,Knipe DM,Cresswell P,Yuan W3.Contrast media and molecular imagingPreclinicalIn VitroandIn VivoEvaluation of [18F]FE@SUPPY for Cancer PET Imaging: Limitations of a Xenograft Model for Colorectal Cancer."14-4732 was used in Immunocytochemistry-immunofluorescence to conclude that HT-29 xenografts are not adequate to visualize hA3ARs using [18F]FE@SUPPY."4.Journal of translational medicineTransforming growth factor-beta 1 produced by vascular smooth muscle cells predicts fibrosis in the gastrocnemius of patients with peripheral artery disease."14-4732 was used in Immunofluorescence to determine the effects of elevated transforming growth factor-beta 1 expression in smooth muscle in response to tissue hypoxia."5.Liver international : official journal of the International Association for the Study of the LiverPhysiological variations of stem cell factor and stromal-derived factor-1 in murine models of liver injury and regeneration."14-4732 was used in IHC/IF to show that changes in stem cell factor and stromal-derived factor-1 varied with the nature of liver injury." |
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