Mouse IgG2b kappa Isotype Control (eBMG2b), PE-eFluor 610
货号：61-4732-80
规格：25 µg
价格：1029
产品类型：流式抗体
品牌：Thermo Fisher
物种：其它
宿主：小鼠
抗体亚型：IgG2b, kappa
荧光染料：Alexa Fluor 488
| 抗体类型 | 单抗 | 同型对照： | |
| 浓度： | 0.2 mg/mL | 用法： | Assay-Dependent (Flow)；Assay-Dependent (Ctrl) |
| 产品详细信息Description: The monoclonal mouse IgG2b is useful as an isotype control immunoglobulin.Applications Reported: Mouse IgG2b K Isotype Control PE-eFluor® 610 has been reported for use in flow cytometric analysis.Applications Tested: Mouse IgG2b K Isotype Control PE-eFluor® 610 has been tested by flow cytometric analysis of normal human peripheral blood cells. Use the isotype control at the same concentration as the experimental antibody.PE-eFluor® 610 can be excited with laser lines from 488-561 nm and emits at 607 nm. We recommend using a 610/20 band pass filter (equivalent to PE-Texas Red®). Please make sure that your instrument is capable of detecting this fluorochome.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 607 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody. |
| 推荐产品： |
| 二抗荧光二抗免疫组化一抗标签抗体 |
©
Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：
https://biorod.cn/thread-27408.htm
QQ:1749072012