Mouse IgG3 Isotype Control (B10), FITC
货号：11-4742-41,11-4742-42
规格：25 Tests,100 Tests
价格：822,1340
产品类型：小鼠同型对照
品牌：eBioscience
物种：其它
宿主：小鼠
抗体亚型：IgG
荧光染料：FITC
| 抗体类型 | 单抗 | 同型对照： | |
| 浓度： | 10 µL/Test | 用法： | 10 μg/test (Flow)；Assay-Dependent (Ctrl) |
| 产品详细信息Description: The mouse IgG3 lambda is useful as an isotype control immunoglobulin.Applications Reported: This mouse IgG3 isotype control has been reported for use in flow cytometric analysis and ELISA.Applications Tested: This mouse IgG3 isotype control has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 10 µL per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. .Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody. |
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| 参考文献： |
| 1.Stem cell researchGeneration of an induced pluripotent stem cell line (TRNDi004-I) from a Niemann-Pick disease type B patient carrying a heterozygous mutation of p.L43_A44delLA in the SMPD1 gene."11-4742 was used in Flow cytometry/Cell sorting to show that this iPSC line offers a useful resource to study the disease pathophysiology and as a cell-based model for drug development to treat NPB."2.Stem cell researchGeneration of two induced pluripotent stem cell lines (NHLBIi001-A and NHLBIi001-B) from a healthy Caucasian female volunteer with normal cardiac function."11-4742 was used in Flow cytometry/Cell sorting to develop two wild-type human-derived induced pluripotent cell lines from a PBMC sample of a patient with normal cardiac function." |
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