Rat IgG1 kappa Isotype Control (eBRG1), Alexa Fluor 647
货号：51-4301-81
规格：50 µg
价格：991
产品类型：大鼠同型对照
品牌：eBioscience
物种：其它
宿主：大鼠
抗体亚型：IgG1, kappa
荧光染料：Alexa Fluor 647
| 抗体类型 | 同型对照 | 同型对照： | |
| 浓度： | 0.2 mg/mL | 用法： | Assay-Dependent (Flow)；Assay-Dependent (Ctrl) |
| 产品详细信息Description: The monoclonal rat IgG1, kappa is useful as an isotype control immunoglobulin.Applications Reported: This rat IgG1 isotype control has been reported for use in flow cytometric analysis.Applications Tested: The Alexa Fluor 647 rat IgG1 isotype control has been tested by flow cytometric analysis of mouse splenocytes and normal human peripheral blood cells and should be used at the same concentration as the experimental antibody.Excitation: 633-647 nm; Emission:668 nm; Laser: Red Laser靶标信息The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody. |
| 数据 |
| Rat IgG1 kappa Isotype Control (51-4301-81) in FlowC57Bl/6 mouse splenocytes were stained with CD4 Monoclonal Antibody, FITC (Product # 11-0042-82) and staining buffer (autofluorescence, left) or 0.125 µg of Rat IgG1 kappa Isotype Control, Alexa Fluor 647 (right). Total cells were used for analysis. |
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