Rat IgG1 kappa Isotype Control (eBRG1), eFluor 450
货号：48-4301-80,48-4301-82
规格：25 μg,100 μg
价格：822,1855
产品类型：大鼠同型对照
品牌：eBioscience
物种：其它
宿主：大鼠
抗体亚型：IgG1, kappa
荧光染料：eFluor 450
| 抗体类型 | 同型对照 | 同型对照： | |
| 浓度： | 0.2 mg/mL | 用法： | Assay-Dependent (Flow)；Assay-Dependent (Ctrl) |
| 产品详细信息Description: The monoclonal rat IgG1, kappa is useful as an isotype control immunoglobulin.Applications Reported: Rat IgG1 Isotype Control eFluor® 450 has been reported for use in flow cytometric analysis.Applications Tested: eFluor® 450 Rat IgG1 Isotype Control has been tested by flow cytometric analysis of mouse splenocytes. Use isotype control at the same concentration as experimental antibody.eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody. |
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| 参考文献： |
| 1.Journal of neuroinflammationChronic thoracic spinal cord injury impairs CD8+ T-cell function by up-regulating programmed cell death-1 expression."48-4301 was used in Flow cytometry/Cell sorting to investigate CD8+ T-cell function by up-regulating programmed cell death-1 expression in chronic thoracic spinal cord injury."2.Bioscience reportsN-terminal pyroglutamate formation in CX3CL1 is essential for its full biologic activity."48-4301 was used in Flow cytometry/Cell sorting to suggest that QC-catalysed N-terminal pGlu formation of CX3CL1 is important for the stability or the interaction with its receptor and opens new insights into the function of QC in inflammation."3.The Journal of biological chemistryGene targeting study reveals unexpected expression of brain-expressed X-linked 2 in endocrine and tissue stem/progenitor cells in mice."17-4301 was used in Flow cytometry/Cell sorting to investigate the precise role and expression pattern of brain-expressed X-linked genes in endocrine and tissue stem/progenitor cells in mice."4.The Journal of biological chemistryGene targeting study reveals unexpected expression of brain-expressed X-linked 2 in endocrine and tissue stem/progenitor cells in mice."17-4301 was used in Flow cytometry/Cell sorting to investigate the precise role and expression pattern of brain-expressed X-linked genes in endocrine and tissue stem/progenitor cells in mice."5.Infection and immunityEfficient B cell responses to Borrelia hermsii infection depend on BAFF and BAFFR but not TACI."17-4301 was used in Flow cytometry/Cell sorting to examine the role of BAFFR and TACI in T cell-independent antibody responses to an active Borrelia hermsii infection." |
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