CD5 Monoclonal Antibody (53-7.3), eFluor 450, eBioscience™

货号：48-0051-80,48-0051-82

规格：25μg,100μg

价格：1237,2580

产品类型：流式抗体

品牌：eBioscience

抗原：CD5

物种：小鼠

宿主：大鼠

抗体亚型：IgG2a, kappa

克隆号： 53-7.3

荧光染料：eFluor 450

抗体类型:	小鼠流式抗体	同型对照：	Rat IgG2a kappa Isotype Control (eBR2a), eFluor 450, eBioscience
浓度：	0.2mg/mL	用法：	0.125 µg/test (Flow)

Description:The 53-7.3 monoclonal antibody reacts with mouse CD5, a 67 kDa protein expressed by a majority of thymocytes, mature T cells and a subset of B cells. The expression of CD5 by a small subset of B cells characterizes a developmentally and functionally distinct lineage of B cells called B-1 cells. CD5 is a counter-receptor for CD72 and plays a role in the T-B cell interaction.Applications Reported: This 53-7.3 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 53-7.3 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.Filtration: 0.2 µm post-manufacturing filtered.Target InformationCD5 is a 67 kDa human T-lymphocyte single-chain transmembrane glycoprotein. CD5 is present on all mature T-lymphocytes, on most of thymocytes and on many T-cell leukemias and lymphomas. CD5 also reacts with a subpopulation of activated B-cells and may act as a receptor in regulating T-cell proliferation. CD5 is found on 95% of thymocytes and 72% of peripheral blood lymphocytes. In lymph nodes, the main reactivity is observed in T cell areas. CD5 is expressed by many T cell leukemia, lymphomas, and activated T cells. Diseases associated with CD5 dysfunction include thymus cancer and Richter's Syndrome.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据：

CD5 Antibody (48-0051-80) in FlowStaining of C57BL/6 splenocytes with Anti-Human/Mouse CD45R (B220) APC-eFluor® 780 (Product # 47-0452-82) and 0.06 µg of Rat IgG2a K Isotype Control eFluor® 450 (Product # 48-4321-82) (left) or 0.06 µg of Anti-Mouse CD5 eFluor® 450 (right). Total viable cells were used for analysis.

推荐产品：

二抗荧光二抗免疫组化一抗标签抗体

参考文献：

1.Arthritis research & therapyMast cell depletion in the preclinical phase of collagen-induced arthritis reduces clinical outcome by lowering the inflammatory cytokine profile."48-0051 was used in Flow cytometry/Cell sorting to suggest that mast cells may play a role in the regulation of the adaptive immune response during the development of arthritis."2.PloS oneNaïve helper T cells with high CD5 expression have increased calcium signaling."Published figure using CD5 monoclonal antibody (Product # 48-0051-82) in Flow Cytometry"3.NatureThemis sets the signal threshold for positive and negative selection in T-cell development."48-0051 was used in Flow cytometry/Cell sorting to show that the T-cell-specific protein Themis can specifically attenuate TCR signal strength via SHP1 recruitment and activation in response to low-affinity TCR engagement."

技术参数

© Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：https://biorod.cn/thread-27456.htm