CD127 Monoclonal Antibody (A7R34), APC-eFluor 780, eBioscience™

货号：47-1271-80,47-1271-82

规格：25μg,100μg

价格：1577,3679

产品类型：流式抗体

品牌：eBioscience

抗原：CD127

物种：小鼠

宿主：大鼠

抗体亚型：IgG2a, kappa

克隆号：A7R34

荧光染料：APC-eFluor 780

抗体类型:	小鼠流式抗体	同型对照：	Rat IgG2a kappa Isotype Control (eBR2a), APC-eFluor 780, eBioscience
浓度：	0.2mg/mL	用法：	0.25 µg/test (Flow)

Description:The A7R34 monoclonal antibody reacts with mouse CD127, the high affinity alpha subunit of the mouse IL-7 receptor. IL-7 receptor alpha chain is expressed by immature B cells in the bone marrow, double-negative (CD4-CD8-), single-positive (CD4+ and CD8+), but not double-positive (CD4+CD8+) thymocytes. In the periphery, mature T cells express CD127 at low level. A7R34 inhibits binding of IL-7 to its receptor and has been used in in vivo and in vitro studies to elucidate the role of IL-7 in T and B cell development and activation. Binding of A7R34 blocks the binding of SB/199, another antibody which recognizes mouse CD127.Applications Reported: This A7R34 antibody has been reported for use in flow cytometric analysis.Applications Tested: This A7R34 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.APC-eFluor 780 emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.Light sensitivity: This tandem is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.Target InformationCD127 (Interleukin-7, IL-7) is a glycoprotein involved in the regulation of lymphopoiesis. The response of cells to CD127 is dependent on the presence of the interleukin 7 receptor (IL7R); the active receptor is an alpha/gamma chain heterodimer. CD127 consists of an alpha chain and a gamma chain. The gamma(c) chain, which also associates with the interleukin-2 receptor, serves primarily to activate signal transduction by the IL7R complex, while the alpha chain of IL7R determines specific signaling events through its association with cytoplasmic signaling molecules. CD127 promotes the proliferation of precursor B cells, thymocytes, T cell progenitors, and mature CD4+ and CD8+ T cells. The biological effects of IL7 are mediated by the binding of IL7 to the specific cell surface receptor. Diseases associated with CD127 dysfunction include severe combined immunodeficiency and T cell negative/B cell negative/NK positive severe combined immunodeficiency.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据：

CD127 Antibody (47-1271-80) in FlowStaining of C57BL/6 splenocytes with Anti-Mouse CD3e FITC (Product # 11-0031-82) and 0.125 µg of Rat IgG2a K Isotype Control APC-eFluor® 780 (Product # 47-4321-82) (left) or 0.125 µg of Anti-Mouse CD127 APC-eFluor® 780 (right). Total viable cells were used for analysis.

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参考文献：

1.The American journal of pathologyFoxp3-expressing CD103+ regulatory T cells accumulate in dendritic cell aggregates of the colonic mucosa in murine transfer colitis.2.Journal of immunology (Baltimore, Md. : 1950)LTbetaR signaling induces cytokine expression and up-regulates lymphangiogenic factors in lymph node anlagen.3.HaematologicaBone marrow adipose tissue-derived stem cell factor mediates metabolic regulation of hematopoiesis."Published figure using CD127 monoclonal antibody (Product # 47-1271-82) in Flow Cytometry"4.ImmunityAn Id2RFP-Reporter Mouse Redefines Innate Lymphoid Cell Precursor Potentials."Published figure using CD127 monoclonal antibody (Product # 47-1271-82) in Flow Cytometry"

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