CD4 Monoclonal Antibody (RM4-5), APC-eFluor 780

货号：47-0042-80,47-0042-82

规格：25 ug,100 μg

价格：934,1959

产品类型：流式抗体

品牌：eBioscience

抗原：CD4

物种：小鼠

宿主：大鼠

抗体亚型：IgG2a, kappa

克隆号：RM4-5

荧光染料：APC-eFluor 780

类型:	单抗	同型对照：	Rat IgG2a kappa Isotype Control (eBR2a), APC-eFluor 780
浓度：	0.2 mg/mL	用法：	0.125 µg/test(Flow)

产品详细信息Description: The RM4-5 monoclonal antibody reacts with the mouse CD4 molecule, a 55 kDa cell surface receptor expressed by a majority of thymocytes, subpopulation of mature T cells and dendritic cells. CD4 binds to MHC class II on the surface of antigen presenting cells and plays an important role both in T cell development and in optimal functioning of mature T cells. In T cells, CD4 associates with protein tyrosine kinase p56lck through its cytoplasmic tail. Binding of RM4-5 is blocked by GK1.5.Applications Reported: This RM4-5 antibody has been reported for use in flow cytometric analysis.Applications Tested: This RM4-5 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.APC-eFluor 780 emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.Light sensitivity: This tandem is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息The CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV. CD4 is primarily expressed in a subset of T-lymphocytes, also referred to as T helper cells, but may also be expressed by other cells in the immune system, such as monocytes, macrophages, and dendritic cells. At the tissue level, CD4 expression may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues. CD4 functions to initiate or augment the early phase of T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase, Lck. It may also function as an important mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcripts have been identified in this gene [RefSeq, July 2017].

数据

CD4 Antibody (47-0042-80) in FlowStaining of BALB/c splenocytes with Anti-Mouse CD3e FITC (Product # 11-0031-82) and 0.06 µg of Rat IgG2a kappa Isotype Control APC-eFluor® 780 (Product # 47-4321-82) (left) or 0.06 µg of Anti-Mouse CD4 APC-eFluor® 780 (right). Total viable cells were used for analysis. CD4 Antibody (47-0042-80)Genome research 2019 -Published figure using CD4 monoclonal antibody (Product # 47-0042-80)

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参考文献

1. Scientific reportsSplenic glucocorticoid resistance following psychosocial stress requires physical injury.2. Journal of immunology (Baltimore, Md. : 1950)Helios Controls a Limited Subset of Regulatory T Cell Functions.3. The Journal of experimental medicineNKT sublineage specification and survival requires the ubiquitin-modifying enzyme TNFAIP3/A20.4. Scientific reportsSystemic inhibition of tissue-nonspecific alkaline phosphatase alters the brain-immune axis in experimental sepsis.5. Genome researchClonal copy-number mosaicism in autoreactive T lymphocytes in diabetic NOD mice.

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