CD127 Monoclonal Antibody (eBioSB/199 (SB/199)), eFluor 450, eBioscience™

货号：48-1273-82

规格：100μg

价格：2987

产品类型：流式抗体

品牌：eBioscience

抗原：CD127

物种：小鼠

宿主：大鼠

抗体亚型：IgG2b, kappa

克隆号：eBioSB/199 (SB/199)

荧光染料：eFluor 450

抗体类型:	小鼠流式抗体	同型对照：	Rat IgG2b kappa Isotype Control (eB149/10H5), eFluor 450, eBioscience
浓度：	0.2 mg/mL	用法：	0.5 µg/test (Flow)

Description:The SB/199 monoclonal antibody reacts with mouse CD127, the high affinity a subunit of the mouse IL-7 receptor. IL-7 receptor a chain is expressed by immature B cells in the bone marrow, double-negative (CD4-/CD8-), single-positive (CD4+ and CD8+), but not double-positive (CD4+/CD8+) thymocytes. In the periphery, mature T cells express low levels of CD127. Binding of SB/199 blocks the binding of A7R34, another antibody which recognizes mouse CD127.Applications Reported: This eBioSB/199 (SB/199) antibody has been reported for use in flow cytometric analysis.Applications Tested: This eBioSB/199 (SB/199) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.Filtration: 0.2 µm post-manufacturing filtered.Target InformationCD127 (Interleukin-7, IL-7) is a glycoprotein involved in the regulation of lymphopoiesis. The response of cells to CD127 is dependent on the presence of the interleukin 7 receptor (IL7R); the active receptor is an alpha/gamma chain heterodimer. CD127 consists of an alpha chain and a gamma chain. The gamma(c) chain, which also associates with the interleukin-2 receptor, serves primarily to activate signal transduction by the IL7R complex, while the alpha chain of IL7R determines specific signaling events through its association with cytoplasmic signaling molecules. CD127 promotes the proliferation of precursor B cells, thymocytes, T cell progenitors, and mature CD4+ and CD8+ T cells. The biological effects of IL7 are mediated by the binding of IL7 to the specific cell surface receptor. Diseases associated with CD127 dysfunction include severe combined immunodeficiency and T cell negative/B cell negative/NK positive severe combined immunodeficiency.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据：

CD127 Antibody (48-1273-82) in FlowStaining of C57Bl/6 splenocytes with Anti-Mouse CD3 APC (Product # 17-0032-82) and 0.25 µg of Rat IgG2b K Isotype Control eFluor® 450 (Product # 48-4031-82) (left) or 0.25 µg of Anti-Mouse CD127 eFluor® 450 (right). Cells in the lymphocyte gate were used for analysis.

推荐产品：

二抗荧光二抗免疫组化一抗标签抗体

参考文献：

1.ImmunitySurvival of Single Positive Thymocytes Depends upon Developmental Control of RIPK1 Kinase Signaling by the IKK Complex Independent of NF-κB."Published figure using CD127 monoclonal antibody (Product # 48-1273-82) in Flow Cytometry"2.Scientific reportsType 2 Innate Lymphocytes Actuate Immunity Against Tumours and Limit Cancer Metastasis."Published figure using CD127 monoclonal antibody (Product # 48-1273-82) in Flow Cytometry"

技术参数

© Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：https://biorod.cn/thread-27511.htm