CD69 Monoclonal Antibody (H1.2F3), APC-eFluor 780, eBioscience™
货号：47-0691-82,47-0691-80
规格：100 µg,25 µg
价格：2432,981
产品类型：流式抗体
品牌：eBioscience
抗原： CD69
物种：小鼠
宿主：Armenian hamster仓鼠
抗体亚型：IgG
克隆号：H1.2F3
荧光染料：APC-eFluor 780
类型: | 流式抗体 | 同型对照： | Armenian Hamster IgG Isotype Control (eBio299Arm), APC-eFluor 780, eBioscience™ |
浓度： | 0.2 mg/mL | 用法： | 0.5 µg/test（Flow） |
产品详细信息Description: The H1.2F3 monoclonal antibody reacts with mouse CD69, also known as very early activation antigen (VEA). CD69 is approximately 35 kDa and is expressed on the surface as a disulfide-linked dimer. While a small subset of lymphocytes in the thymus, spleen and lymph nodes express this antigen, activation of both T and B cells rapidly upregulates the surface expression of CD69, suggesting a role for CD69 in lymphocyte development and activation.Applications Reported: This H1.2F3 antibody has been reported for use in flow cytometric analysis.Applications Tested: This H1.2F3 antibody has been tested by flow cytometric analysis of stimulated mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.APC-eFluor 780 emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.Light sensitivity: This tandem is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD69 (AIM, Active Inducer Molecule) is a gp28/34 disulfide bonded homodimer with a molecular weight of 60 kDa under non-reducing conditions. CD69 contains one or two N linked oligosacaride and the molecule is present on activated platelets. In normal peripheral blood a variable percentage of cells express the CD69 antigen, and it is involved in lymphocyte signal transduction. Expression CD69 is induced upon activation of T lymphocytes, and may play a role in proliferation. Furthermore, the protein may act to transmit signals in natural killer cells and platelets. Alternative splicing results in multiple transcript variants of CD69. Diseases associated with CD69 dysfunction include coccidiodomycosis and asthma.仅用于科研。不用于诊断过程。
数据 |
| CD69 Antibody (47-0691-82) in FlowStaining of 1 day Con A-stimulated C57Bl/6 splenocytes with Anti-Mouse CD25 Alexa Fluor® 488 (Product # 53-0251-82) and 0.25 µg of Armenian Hamster IgG Isotype Control APC-eFluor® 780 (Product # 47-4888-80) (left) or 0.25 µg of Anti-Mouse CD69 APC-eFluor® 780 (right). Cells in the lymphocyte gate were used for analysis. |
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参考文献： |
| 1. NPJ vaccinesEnhanced germinal center reaction by targeting vaccine antigen to major histocompatibility complex class II molecules."Published figure using CD69 monoclonal antibody (Product # 47-0691-82) in Flow Cytometry"Year2020SpeciesArtificial Control2. Protein & cellContact-dependent delivery of IL-2 by dendritic cells to CD4 T cells in the contraction phase promotes their long-term survival."Published figure using CD69 monoclonal antibody (Product # 47-0691-82) in Flow Cytometry"Year2020SpeciesArtificial Control3. Cell communication and signaling : CCSTargeting the orphan nuclear receptor NR2F6 in T cells primes tumors for immune checkpoint therapy."Published figure using CD69 monoclonal antibody (Product # 47-0691-82) in Flow Cytometry"Year2020SpeciesArtificial Control4. Experimental and therapeutic medicineIncrease in liver γδ T cells with concurrent augmentation of IFN-β production during the early stages of a mouse model of acute experimental hepatitis B virus infection."Published figure using CD69 monoclonal antibody (Product # 47-0691-82) in Flow Cytometry"Year20205. Journal of neuroinflammationCSF1R antagonism limits local restimulation of antiviral CD8+T cells during viral encephalitis."Published figure using CD69 monoclonal antibody (Product # 47-0691-82) in Flow Cytometry"Year2019SpeciesArtificial Control |
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