CD8a Monoclonal Antibody (53-6.7), Alexa Fluor 700
货号：56-0081-80,56-0081-82
规格：25 μg,100 μg
价格：1237,2223
产品类型：流式抗体
品牌：Thermo Fisher
物种：人
宿主：小鼠
抗体亚型：IgG2a, kappa
荧光染料：Alexa Fluor 700
| 抗体类型 | 一抗 | 同型对照： | Rat IgG2a kappa Isotype Control (eBR2a), Alexa Fluor 700 |
| 浓度： | 0.2 mg/mL | 用法： | 0.125 μg/test (Flow)； |
| 产品详细信息Description: The 53-6.7 monoclonal antibody reacts with the mouse CD8a molecule. CD8a is an approximately 32-34 kDa cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha beta) or as a homodimer (CD8 alpha alpha). A majority of thymocytes and a subpopulation of mature alpha beta TCR T cells express CD8 alpha beta while gamma delta TCR T cells, a subpopulation of intestinal intraepithelial lymphocytes (IELs) and dendritic cells express CD8 alpha alpha. CD8 binds to MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T cell development and activation of mature T cells.Applications Reported: This 53-6.7 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 53-6.7 antibody has been tested by flow cytometric analysis of mouse thymocyte and splenocyte suspensions. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Alexa Fluor® 700 emits at 723 nm and can be excited with the red laser (633 nm). Most instruments will require a 685 LP mirror and 710/20 filter. Please make sure that your instrument is capable of detecting this fluorochrome.Excitation: 633-647 nm; Emission: 723 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD8 molecule is composed of two chains termed alpha and beta. CD8 is found on a T cell subset of normal cytotoxic / suppressor cells which make up approximately 20 to 35% of human peripheral blood lymphocytes. The CD8 antigen is also detected on natural killer cells, 80% of thymocytes, on a subpopulation of 30% of peripheral blood null cells and 15 to 30% of bone marrow cells. |
| 数据 |
| CD8a Antibody (56-0081-80) in FlowStaining of C57BL/6 splenocytes with staining buffer (autofluorescence) (open histogram) or 0.06 µg of Anti-Mouse CD8a Alexa Fluor® 700 (filled histogram). Cells in the lymphocyte gate were used for analysis. |
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| 参考文献： |
| 1.Scientific reportsDiscovery of a Metastatic Immune Escape Mechanism Initiated by the Loss of Expression of the Tumour Biomarker Interleukin-33."Published figure using CD8a monoclonal antibody (Product # 56-0081-82) in Flow Cytometry"2.Scientific reportsType 2 Innate Lymphocytes Actuate Immunity Against Tumours and Limit Cancer Metastasis."Published figure using CD8a monoclonal antibody (Product # 56-0081-82) in Immunohistochemistry"3.OncotargetUse of tilapia piscidin 3 (TP3) to protect against MRSA infection in mice with skin injuries."Published figure using CD8a monoclonal antibody (Product # 56-0081-82) in Immunohistochemistry"4.American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant SurgeonsImaging Tolerance Induction in the Classic Medawar Neonatal Mouse Model: Active Roles of Multiple F1-Donor Cell Types."Published figure using CD8a monoclonal antibody (Product # 56-0081-82) in Immunocytochemistry"5.Nature communicationsOncofetal gene SALL4 reactivation by hepatitis B virus counteracts miR-200c in PD-L1-induced T cell exhaustion."Published figure using CD8a monoclonal antibody (Product # 56-0081-82) in Immunofluorescence" |
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