CD69 Monoclonal Antibody (H1.2F3), Functional Grade, eBioscience™

货号：16-0691-85,16-0691-38,16-0691-025,16-0691-050

规格：50 µg,5 mg,25 mg,50 mg

价格：3434,24089,72266,120443

产品类型：流式抗体

品牌：eBioscience

抗原：CD69

物种：小鼠

宿主：Armenian hamster仓鼠

抗体亚型：IgG

克隆号：H1.2F3

荧光染料：Purified

类型:	一抗	同型对照：	Armenian Hamster IgG Isotype Control (eBio299Arm), Functional Grade, eBioscience™
浓度：	1 mg/mL	用法：	0.5 µg/test（Flow）

产品详细信息Description: The H1.2F3 monoclonal antibody reacts with mouse CD69, also known as very early activation antigen (VEA). CD69 is approximately 35 kDa and is expressed on the surface as a disulfide-linked dimer. While a small subset of lymphocytes in the thymus, spleen and lymph nodes express this antigen, activation of both T and B cells rapidly upregulates the surface expression of CD69, suggesting a role for CD69 in lymphocyte development and activation.Applications Reported: The H1.2F3antibody has been reported for use in flow cytometric analysis. H1.2F3 has also been reported in in vitro cell activation.Applications Tested: The H1.2F3 antibody has been tested by flow cytometric analysis of resting and activated mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.靶标信息CD69 (AIM, Active Inducer Molecule) is a gp28/34 disulfide bonded homodimer with a molecular weight of 60 kDa under non-reducing conditions. CD69 contains one or two N linked oligosacaride and the molecule is present on activated platelets. In normal peripheral blood a variable percentage of cells express the CD69 antigen, and it is involved in lymphocyte signal transduction. Expression CD69 is induced upon activation of T lymphocytes, and may play a role in proliferation. Furthermore, the protein may act to transmit signals in natural killer cells and platelets. Alternative splicing results in multiple transcript variants of CD69. Diseases associated with CD69 dysfunction include coccidiodomycosis and asthma.仅用于科研。不用于诊断过程。

数据

CD69 Antibody (16-0691-85) in FlowStaining of ConA stimulated splenocytes with Anti-Mouse CD69 PE. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.

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参考文献：

1.Experimental and therapeutic medicineIncrease in liver γδ T cells with concurrent augmentation of IFN-β production during the early stages of a mouse model of acute experimental hepatitis B virus infection."Published figure using CD69 monoclonal antibody (Product # 16-0691-85) in Flow Cytometry"2.Scientific reportsDevelopment of a screening strategy for new modulators of T cell receptor signaling and T cell activation."Published figure using CD69 monoclonal antibody (Product # 16-0691-85) in Flow Cytometry"3.Scientific reportsHomoharringtonine induced immune alteration for an Efficient Anti-tumor Response in Mouse Models of Non-small Cell Lung Adenocarcinoma Expressing Kras Mutation."Published figure using CD69 monoclonal antibody (Product # 16-0691-85) in Flow Cytometry"4.Nature communicationsOncofetal gene SALL4 reactivation by hepatitis B virus counteracts miR-200c in PD-L1-induced T cell exhaustion."Published figure using CD69 monoclonal antibody (Product # 16-0691-85) in Flow Cytometry"5.Clinical and experimental immunologyMicroRNA-126 deficiency enhanced the activation and function of CD4+T cells by elevating IRS-1 pathway."Published figure using CD69 monoclonal antibody (Product # 16-0691-85) in Flow Cytometry

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