CD27 Monoclonal Antibody (LG.7F9), PE-Cyanine7, eBioscience™

货号：25-0271-82,25-0271-80

规格：100 µg,25 µg

价格：3341,1416

产品类型：流式抗体

品牌：eBioscience

抗原：CD27

物种：人/小鼠/大鼠

宿主：Armenian hamster仓鼠

抗体亚型：IgG

克隆号：LG.7F9

荧光染料：PE-Cy7

类型:	一抗	同型对照：	Armenian Hamster IgG Isotype Control (eBio299Arm), PE-Cyanine7, eBioscience™
浓度：	0.2 mg/mL	用法：	0.5 µg/test（Flow）

产品详细信息Description: The LG.7F9 monoclonal antibody reacts with mouse CD27, a lymphocyte-specific member of the TNFR superfamily. CD27 is expressed by virtually all mature T cells and by a subpopulation of B cells, mainly memory B cells. In mouse, CD27 has been found on nearly all thymocytes excluding a population of CD46^-CD8^- precursors. CD27 binds to CD70 and, through this interaction, plays an important role in T cell-B cell interaction. It has been reported that triggering CD27 plays an importantrole in the maturation of CD4^+ and CD8^+ effector cells. LG.7F9 cross-reacts with human and rat CD27.Applications Reported: This LG.7F9 antibody has been reported for use in flow cytometric analysis.Applications Tested: This LG.7F9 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD27 is a 50 kDa member of the tumor necrosis factor (TNF) receptor superfamily that includes CD40 and CD30. The TNF superfamily members are known for the regulation of cell proliferation and death. In contrast to the expression of other TNFR/TNF family members, expression of CD27 and its ligand CD70 is predominantly confined to lymphocytes. High expression levels of CD27 appear to be dependent on proper ligation of antigen receptors. CD70 expression requires additional co-stimulatory and/or pro-inflammatory signals. CD27 is expressed as a disulfide-linked homodimer on mature thymocytes, peripheral blood T cells and a subpopulation of B cells. Activation of T cells via TCR-CD3 complex results in upregulation of CD27 expression on the plasma membrane as well as in the release of its soluble 28-32 kDa form, sCD27, detected in the plasma, urine or spinal fluid. Soluble CD27 is an important prognostic marker of acute and chronic B cell malignancies. RgpA, a cystein proteinase, although activating T cells through the protease-activated receptors (PARs), degradates CD27 and counteracts T cell activation mediated by CD27 and its ligand CD70. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor. Diseases associated with CD27 dysfunction include Lymphoproliferative Syndrome 2 and Autosomal Recessive Lymphoproliferative Syndrome.仅用于科研。不用于诊断过程。

数据

CD27 Antibody (25-0271-82) in FlowStaining of C57BL/6 splenocytes with Anti-Human/Mouse CD45R (B220) FITC (Product # 11-0452-82) and 0.25 µg of Armenian Hamster IgG Isotype Control PE-Cyanine7 (Product # 25-4888-82) (left) or 0.25 µg of Anti-CD27 PE-Cyanine7 (right). Total viable cells were used for analysis.

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参考文献：

1. Cancer immunology researchPD-1+Tim-3+ CD8+ T Lymphocytes Display Varied Degrees of Functional Exhaustion in Patients with Regionally Metastatic Differentiated Thyroid Cancer."25-0271 was used in Flow cytometry/Cell sorting to determine that the release of PD-1 and Tim-3, and the inhibition of Tregs, may encourage tumour elimination in metastatic differentiated thyroid cancer."AuthorsSeverson JJ,Serracino HS,Mateescu V,Raeburn CD,McIntyre RC,Sams SB,Haugen BR,French JDYear2015SpeciesHuman2. European journal of immunologyActivated NKT cells imprint NK-cell differentiation, functionality and education."25-0271 was used in Flow cytometry/Cell sorting to study the cellular cross-talk between natural killer T cells and natural killer cells."AuthorsRiese P,Trittel S,May T,Cicin-Sain L,Chambers BJ,Guzmán CAYear2015SpeciesMouse3. Journal of virologyType I interferon signaling enhances CD8+ T cell effector function and differentiation during murine gammaherpesvirus 68 infection."25-0271 was used in Flow cytometry/Cell sorting to assess T cell responses in IFNAR1(-/-) mice after MHV68 infection."AuthorsJennings RN,Grayson JM,Barton ESYear2014SpeciesMouseDilution1:1004. Nature immunologyThe tyrosine phosphatase PTPN22 discriminates weak self peptides from strong agonist TCR signals."25-0271 was used in Flow cytometry/Cell sorting to study how loss-of-function PTPN22 alleles can lead to the population expansion of effector and/or memory T cells and a predisposition to human autoimmunity."AuthorsSalmond RJ,Brownlie RJ,Morrison VL,Zamoyska RYear2014SpeciesMouse5. Journal of immunology (Baltimore, Md. : 1950)Human γδ thymocytes are functionally immature and differentiate into cytotoxic type 1 effector T cells upon IL-2/IL-15 signaling."25-0271 was used in Flow cytometry/Cell sorting to study how ex vivo-isolated γδ thymocytes produced negligible IFN-γ and lacked cytolytic activity against leukemia cells."AuthorsRibot JC,Ribeiro ST,Correia DV,Sousa AE,Silva-Santos B

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