CD25 Monoclonal Antibody (PC61.5), PE-Cyanine5, eBioscience™

货号：15-0251-82

规格：100 µg

价格：2683

产品类型：流式抗体

品牌：eBioscience

物种：小鼠

宿主：大鼠

抗体亚型：其它

荧光染料：PE-Cy5

抗体类型:	小鼠流式抗体	同型对照：	Rat IgG1 kappa Isotype Control (eBRG1), PE-Cyanine5, eBioscience
浓度：	0.2 mg/mL	用法：	0.125 µg/test (Flow)

Description:The PC61.5 antibody reacts with mouse CD25, the 55 kDa interleukin-2 receptor alpha chain (IL-2R alpha). CD25 is expressed by early progenitors of the T and B lineage as well as by activated mature T and B lymphocytes. By itself, CD25 binds IL-2 only with low affinity. However, CD25 associates with CD122 (IL-2 receptor beta chain) and CD132 (common gamma chain) to form the high affinity IL-2 receptor. Binding of IL-2 to both the high and low affinity classes of IL-2 receptor is inhibited by the PC61.5 antibody. CD25 plays a role in lymphocyte differentiation and activation/proliferation.Applications Reported: The PC61.5 antibody has been reported for use in flow cytometric analysis.Applications Tested: The PC61.5 antibody has been tested by flow cytometric analysis of mouse splenocytes and Con A-stimulated splenocyte culture. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 667 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.Target InformationCD25 (IL2 receptor alpha chain/IL2RA) is a cytokine that plays a role in the proliferation of T and B lymphocytes. The receptor of this cytokine (IL2RA) is a heterotrimeric protein complex with a gamma chain also shared by interleukin 4 (IL4) and interleukin 7 (IL7). IL2RA, IL2R beta chain (IL2RB), and the IL2R gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric IL2RA chains result in low-affinity receptor, while homodimeric IL2RB chains produce a medium-affinity receptor. The expression of IL2 in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. IL2 is primarily produced by mature T cells. IL2 plays an important role as a growth factor, differentiation factor, and regulator of cell death. IL-2 stimulates the proliferation of B cells, augments natural killer cell activity, and inhibits granulocyte macrophage colony formation. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests a role in the immune response to antigenic stimuli. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据：

CD25 Antibody (15-0251-82)Staining of stimulated mouse splenocytes. As expected based on known expression patterns, CD25 clone PC61.5 staining is significantly increased in CD8+ T cells after stimulation. Details: Mouse splenocytes were unstimulated (blue histogram) or stimulated for 2 days with CD3 (clone 145-2C11), CD28 (clone 37.51), and Mouse IL-2 Recombinant Protein (purple histogram). After stimulation, cells were stained with CD8 (clone 53-6.7) and CD25 (clone PC61.5). Cells in the CD8+ gate were used for analysis.

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参考文献：

1.Frontiers in immunologyRole of Nuclear Factor (Erythroid-Derived 2)-Like 2 Signaling for Effects of Fumaric Acid Esters on Dendritic Cells."15-0251 was used in Flow cytometry/Cell sorting to reveal, using treatment with fumaric acid esters as a model, the effects of Nrf2 activation and signalling on dendritic cells."2.PloS oneGlutamic acid decarboxylase-derived epitopes with specific domains expand CD4(+)CD25(+) regulatory T cells."15-0251 was used in Flow cytometry/Cell sorting to investigate how structural analysis of epitopes may aid predictions of protective epitopes to expand functional Tregs."3.Cancer researchCombination of tumor site-located CTL-associated antigen-4 blockade and systemic regulatory T-cell depletion induces tumor-destructive immune responses.4.Cancer researchListeria monocytogenes promotes tumor growth via tumor cell toll-like receptor 2 signaling.

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