CD154 (CD40 Ligand) Monoclonal Antibody (MR1), APC, eBioscience™

货号：17-1541-81,17-1541-82

规格：50 µg,100 µg

价格：1267,2312

产品类型：流式抗体

品牌：eBioscience

抗原：CD154

物种：小鼠

宿主：Armenian hamster仓鼠

抗体亚型：IgG

克隆号：MR1

荧光染料：APC

类型:	一抗	同型对照：	Armenian Hamster IgG Isotype Control (eBio299Arm), APC, eBioscience™
浓度：	0.2 mg/mL	用法：	0.25 µg/test（Flow）

产品详细信息Description: The MR1 monoclonal antibody reacts with mouse CD154, a 39 kDa transmembrane glycoprotein also known as CD40 ligand (CD40L). CD154 is expressed transiently by activated T cells. Through its binding to CD40 on antigen presenting cells (APC) including B cells, monocytes/macrophages, and dendritic cells, it serves a crucial function in T cell-APC cognate interaction. CD154-interaction with CD40 transduces signals for T-dependent B cell activation and induces B cells to enter the cell cycle.For staining for flow cytometric analysis, it is important to stimulate enriched T cells or enriched CD4 cells (using depletion strategy) prior to staining with MR1.Applications Reported: This MR1 antibody has been reported for use in flow cytometric analysis.Applications Tested: This MR1 antibody has been tested by flow cytometric analysis of stimulated mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD40 Ligand (CD40-L), or CD154, is a membrane glycoprotein and differentiation antigen expressed on the surface of T cells. The CD40 Ligand stimulates B cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines. CD40 Ligand has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. It also co-stimulates proliferation of activated T cells and this is accompanied by the production of IFN-gamma, TNF-alpha, and IL-2.仅用于科研。不用于诊断过程。

数据

CD154 (CD40 Ligand) Antibody (17-1541-81) in FlowT cells sorted from BALB/c mouse splenocytes using the MagniSort™ Mouse T cell Enrichment Kit (Product # 8804-6820-74) were stimulated overnight with the Cell Stimulation Cocktail (Product # 00-4970-03) and then stained with CD4 Monoclonal Antibody, FITC (Product # 11-0041-82) and 0.125 µg of Armenian Hamster IgG Isotype Control, APC (Product # 17-4888-82) (left) or 0.125 µg of CD154 Monoclonal Antibody, APC (right). Total viable cells were used for analysis, as determined by Fixable Viability Dye eFluor 450 (Product # 65-0863-18).

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参考文献：

1. Nature communicationsγδ T cells control humoral immune response by inducing T follicular helper cell differentiation."17-1541 was used in Flow cytometry/Cell sorting to demonstrate that complete Freund's adjuvant immunisation induces a subpopulation of CXCR5-expressing γδ T cells in the draining lymph nodes."AuthorsRezende RM,Lanser AJ,Rubino S,Kuhn C,Skillin N,Moreira TG,Liu S,Gabriely G,David BA,Menezes GB,Weiner HLYear2018SpeciesMouse 2. PloS oneThe tuberculosis vaccine candidate Bacillus Calmette-Guérin ΔureC::hly coexpressing human interleukin-7 or -18 enhances antigen-specific T cell responses in mice."17-1541 was used in Flow cytometry/Cell sorting to determine whether vaccine strains which express interleukin-7 (hIL)-7 or hIL-18 in the genetic background of BCG δureC::hly modulate specific T cell immunity."AuthorsRao M,Vogelzang A,Kaiser P,Schuerer S,Kaufmann SH,Gengenbacher MYear2014SpeciesMouse 3. PloS oneSurveillance on the status of immune cells after Echinnococcus granulosus protoscoleces infection in Balb/c mice."17-1541 was used in Flow cytometry/Cell sorting to investigate the systemic immune status of innate and adaptive immunity at 30, 180, 360 days post-infection in mice infected with E. granulousus."AuthorsPan W,Zhou HJ,Shen YJ,Wang Y,Xu YX,Hu Y,Jiang YY,Yuan ZY,Ugwu CE,Cao JPYear2014SpeciesMouse 4. BloodCD4+ T-cell epitopes associated with antibody responses after intravenously and subcutaneously applied human FVIII in humanized hemophilic E17 HLA-DRB1*1501 mice."17-1541 was used in Flow cytometry/Cell sorting to identify FVIII peptides presented by HLA-DRB1*1501."AuthorsSteinitz KN,van Helden PM,Binder B,Wraith DC,Unterthurner S,Hermann C,Schuster M,Ahmad RU,Weiller M,Lubich C,de la Rosa M,Schwarz HP,Reipert BMYear2012SpeciesMouse 5. BMC immunologyDX5+NKT cells display phenotypical and functional differences between spleen and liver as well as NK1.1-Balb/c and NK1.1+ C57Bl/6 mice."17-1541 was used in Flow cytometry/Cell sorting to investigate the expression of several T cell and NK cell markers in spleen and liver DX5+NKT cell subsets in mice."AuthorsWerner JM,Busl E,Farkas SA,Schlitt HJ,Geissler EK,Hornung M

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