CD154 (CD40 Ligand) Monoclonal Antibody (MR1), eFluor 450, eBioscience™

货号：48-1541-82

规格：100 µg

价格：2476

产品类型：流式抗体

品牌：eBioscience

抗原：CD154

物种：小鼠

宿主：Armenian hamster仓鼠

抗体亚型：IgG

克隆号：eFluor® 450

荧光染料：eFluor 450

类型:	一抗	同型对照：	Armenian Hamster IgG Isotype Control (eBio299Arm), eFluor 450, eBioscience™
浓度：	0.2 mg/mL	用法：	0.5 µg/test（Flow）

产品详细信息Description: The MR1 monoclonal antibody reacts with mouse CD154, a 39 kDa transmembrane glycoprotein also known as CD40 ligand (CD40L). CD154 is expressed transiently by activated T cells. Through its binding to CD40 on antigen presenting cells (APC) including B cells, monocytes/macrophages, and dendritic cells, it serves a crucial function in T cell-APC cognate interaction. CD154-interaction with CD40 transduces signals for T-dependent B cell activation and induces B cells to enter the cell cycle.For staining for flow cytometric analysis, it is important to stimulate enriched T cells or enriched CD4 cells (using depletion strategy) prior to staining with MR1.Applications Reported: This MR1 antibody has been reported for use in flow cytometric analysis.Applications Tested: This MR1 antibody has been tested by flow cytometric analysis of stimulated mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD40 Ligand (CD40-L), or CD154, is a membrane glycoprotein and differentiation antigen expressed on the surface of T cells. The CD40 Ligand stimulates B cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines. CD40 Ligand has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. It also co-stimulates proliferation of activated T cells and this is accompanied by the production of IFN-gamma, TNF-alpha, and IL-2.仅用于科研。不用于诊断过程。

数据

CD154 (CD40 Ligand) Antibody (48-1541-82) in FlowT cells sorted from BALB/c splenocytes using the MagniSort™ Mouse T cell Enrichment Kit (Product # 8804-6820-74) were unstimulated (orange histogram) or stimulated with the Cell Stimulation Cocktail (Product # 00-4970-03) (purple histogram) and stained with 0.25 µg of Armenian Hamster IgG Isotype Control eFluor® 450 (Product # 48-4888-82) (left) or 0.25 µg of Anti-Mouse CD154 (CD40 Ligand) eFluor® 450 (right). Cells in the lymphocyte gate were used for analysis.

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