CD154 (CD40 Ligand) Monoclonal Antibody (MR1), PerCP-eFluor 710, eBioscience™

货号：46-1541-80,46-1541-82

规格：25 µg,100 µg

价格：1164,2630

产品类型：流式抗体

品牌：eBioscience

抗原：CD154

物种：小鼠

宿主：Armenian hamster仓鼠

抗体亚型：IgG

克隆号：MR1

荧光染料：PerCP-eFluor™ 710

类型:	一抗	同型对照：	Armenian Hamster IgG Isotype Control (eBio299Arm), PerCP-eFluor 710, eBioscience™
浓度：	0.2 mg/mL	用法：	0.125 µg/test（Flow）

产品详细信息Description: The MR1 monoclonal antibody reacts with mouse CD154, a 39 kDa transmembrane glycoprotein also known as CD40 ligand (CD40L). CD154 is expressed transiently by activated T cells. Through its binding to CD40 on antigen presenting cells (APC) including B cells, monocytes/macrophages, and dendritic cells, it serves a crucial function in T cell-APC cognate interaction. CD154-interaction with CD40 transduces signals for T-dependent B cell activation and induces B cells to enter the cell cycle.For staining for flow cytometric analysis, it is important to stimulate enriched T cells or enriched CD4 cells (using depletion strategy) prior to staining with MR1.Applications Reported: This MR1 antibody has been reported for use in flow cytometric analysis.Applications Tested: This MR1 antibody has been tested by flow cytometric analysis of stimulated mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD40 Ligand (CD40-L), or CD154, is a membrane glycoprotein and differentiation antigen expressed on the surface of T cells. The CD40 Ligand stimulates B cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines. CD40 Ligand has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. It also co-stimulates proliferation of activated T cells and this is accompanied by the production of IFN-gamma, TNF-alpha, and IL-2.仅用于科研。不用于诊断过程。

数据

CD154 (CD40 Ligand) Antibody (46-1541-82) in FlowT cells sorted from BALB/c splenocytes using the MagniSort™ Mouse T cell Enrichment Kit (Product # 8804-6820-74) were unstimulated (orange histogram) or stimulated with the Cell Stimulation Cocktail (Product # 00-4970-03) (purple histogram) and stained with 0.06 µg of Armenian Hamster IgG Isotype Control PerCP-eFluor® 710 (Product # 46-4888-82) (left) or 0.06 µg of Anti-Mouse CD154 (CD40 Ligand) PerCP-eFluor® 710 (right). Cells in the lymphocyte gate were used for analysis.

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参考文献：

1.Proceedings of the National Academy of Sciences of the United States of AmericaNaive CD4 T cells constitutively express CD40L and augment autoreactive B cell survival.2.NPJ vaccinesReprogramming the adjuvant properties of aluminum oxyhydroxide with nanoparticle technology."46-1541 was used in Flow cytometry/Cell sorting to determine whether the particle size and aggregated state of aluminum oxyhydroxide affects its adjuvant activity."AuthorsOrr MT,Khandhar AP,Seydoux E,Liang H,Gage E,Mikasa T,Beebe EL,Rintala ND,Persson KH,Ahniyaz A,Carter D,Reed SG,Fox CB

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