CD20 Monoclonal Antibody (2H7), eFluor 450, eBioscience
货号:48-0209-41,48-0209-42
规格:25 tests,100 tests
价格:1244,2794
产品类型:流式抗体
品牌:eBioscience
抗原:CD20
物种:人
宿主:小鼠
抗体亚型:IgG2b, kappa
克隆号:2H7
荧光染料:eFluor 450
抗体类型: | 人CD20细胞流式抗体 | 同型对照: | Mouse IgG2b kappa Isotype Control (eBMG2b), eFluor 450, eBioscience |
浓度: | 5 μL/Test | 用法: | 5μL(0.5 µg)/test (Flow) |
Description:The 2H7 monoclonal antibody reacts with human CD20, a 33-36 kDa transmembrane protein. CD20 is expressed by developing B cells as well as mature B cells but not plasma cells. CD20 has been detected at low levels on a small subset of mature T cells. It is suggested that CD20 plays a role in B-cell activation.Applications Reported: This 2H7 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 2H7 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.Filtration: 0.2 µm post-manufacturing filtered.Target InformationCD20 is a non-glycosylated surface phosphoprotein that has a molecular weight range of 33-37 kDa depending on the degree of phosphorylation. CD20 is expressed on mature and most malignant B cells, in a subpopulation of T lymphocytes and follicular dendritic cells. CD20 expression on B cells is synchronous with the expression of surface IgM and it regulates transmembrane calcium conductance, cell cycle progression and B-cell proliferation. CD20 is also associated with lipid rafts, but the intensity of this association depends on extracellular triggering, employing CD20 conformational change, and/or BCR (B cell antigen receptor) aggregation. After the receptor ligation, BCR and CD20 colocalize and then rapidly dissociate before BCR endocytosis, whereas CD20 remains at the cell surface. CD20 serves as a useful target for antibody-mediated therapeutic depletion of B cells, as it is expressed at high levels on most B-cell malignancies, but does not become internalized or shed from the plasma membrane following monoclonal antibody treatment. Diseases associated with CD20 dysfunction include Ms4a1-related common variable immune deficiency.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
数据: |
CD20 Antibody (48-0209-41) in FlowStaining of normal human peripheral blood cells with Anti-Human CD3 APC (Product # 17-0038-42) and Mouse IgG2b K Isotype Control eFluor® 450 (Product # 48-4732-82) (left) or Anti-Human CD20 eFluor® 450 (right). Cells in the lymphocyte gate were used for analysis. |
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参考文献: |
1.PloS oneSexual Differences in Cell Loss during the Post-Hatch Development of Song Control Nuclei in the Bengalese Finch."Published figure using CD20 monoclonal antibody (Product # 48-0209-42) in Immunohistochemistry"2.Nature communicationsTumor-associated B-cells induce tumor heterogeneity and therapy resistance."Published figure using CD20 monoclonal antibody (Product # 48-0209-42) in Flow Cytometry"3.Frontiers in immunologyCLEC10A Is a Specific Marker for Human CD1c+Dendritic Cells and Enhances Their Toll-Like Receptor 7/8-Induced Cytokine Secretion."48-0209 was used in Flow cytometry/Cell sorting to identify CLEC10A as a specific marker for human CD1c+ dendritic cells and that this rapidly internalises into human CD1c dendritic cells upon binding of a monoclonal antibody directed against CLEC10A."4.Nature communicationsEvidence for persistence of the SHIV reservoir early after MHC haploidentical hematopoietic stem cell transplantation."Published figure using CD20 monoclonal antibody (Product # 48-0209-42) in Flow Cytometry" |
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