CD80 (B7-1) Monoclonal Antibody (2D10.4), PE-Cyanine5, eBioscience

货号：15-0809-42

规格：100 Tests

价格：2947

产品类型：流式抗体

品牌：eBioscience

抗原：CD80

物种：人

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：2D10.4

荧光染料：PE-Cy5

类型:	一抗	同型对照：	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE-Cyanine5, eBioscience
浓度：	5 µL/Test	用法：	5 µL (1 µg)/test

产品详细信息Description: The 2D10.4 monoclonal antibody reacts with human CD80 (B7-1), a 60 kDa member of the Ig superfamily. CD80 is expressed by activated B cells, macrophages and dendritic cells. In addition, activated T cells express this antigen. CD80 has high affinity for binding to two T cell surface antigens, CD28 and CD152 (CTLA-4). The interaction of CD28 and CD152 with CD80 is crucial in T-B cell communication leading to activation of T and B cells, respectively.Applications Reported: The 2D10.4 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 2D10.4 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (1 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 667 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD80 (B7-1) and CD86 (B7-2) are ligands of T cell critical costimulatory molecule CD28, and of an inhibitory receptor CTLA-4 (CD152). Both B7 molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, and both molecules can also substitute for each other in this process. CD80 is rapidly induced on the surface of in vitro activated B cells, with Epstein Barr Virus (EBV) transformed B cell lines, with Burkitts lymphoma cell lines, with freshly isolated follicular B lymphoma cells, T cells, and monocytes. The B-lymphocyte activation antigen B7-1 provides regulatory signals for T lymphocytes as a consequence of binding to the CD28 and CTLA4 ligands of T cells. Diseases associated with CD80 dysfunction include gallbladder squamous cell carcinoma and myocarditis.仅用于科研。不用于诊断过程。

数据

CD80 (B7-1) Antibody (15-0809-42) in FlowStaining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control PE-Cyanine5 (Product # 15-4714-81) (open histogram) or Anti-Human CD80 (B7-1) PE-Cyanine5 (filled histogram).Cells in the lymphocyte gate were used for analysis.

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参考文献：

1. NutrientsAstragalus polysaccharides(PG2) Enhances the M1 Polarization of Macrophages, Functional Maturation of Dendritic Cells, and T Cell-Mediated Anticancer Immune Responses in Patients with Lung Cancer."Published figure using CD80 (B7-1) monoclonal antibody (Product # 15-0809-42) in Flow Cytometry"2. Oncology lettersPhotodynamic therapy reduces the inhibitory effect of osteosarcoma cells on dendritic cells by upregulating HSP70."Published figure using CD80 (B7-1) monoclonal antibody (Product # 15-0809-42) in Flow Cytometry"3. PloS oneA Francisella tularensis live vaccine strain that improves stimulation of antigen-presenting cells does not enhance vaccine efficacy."15-0809 was used in Flow cytometry/Cell sorting to examine whether the interaction of genetically modified Francisella tularensis live vaccine strains with human antigen-presenting cells correlates with vaccine effectiveness."AuthorsSchmitt DM,O'Dee DM,Horzempa J,Carlson PE,Russo BC,Bales JM,Brown MJ,Nau GJ

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