CD24 Monoclonal Antibody (eBioSN3 (SN3 A5-2H10)),

货号：14-0247-82

规格：100 ug

价格：1283

产品类型：流式抗体

品牌：eBioscience

抗原：CD24

物种：人

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：eBioSN3 (SN3 A5-2H10

荧光染料：其它

类型:	单抗	同型对照：
浓度：	0.5 mg/mL	用法：	0.5 µg/test(Flow)

产品详细信息Description: The SN3 monoclonal antibody reacts with the human CD24 molecule, also known as Heat Stable Antigen (HSA). This 35-50 kDa molecule is anchored in the plasma membrane via phosphatidylinositol and is expressed by erythrocytes, thymocytes, peripheral lymphocytes and myeloid lineage. It has been reported that P-selectin (CD62P) binds to CD24.Applications Reported: The SN3 antibody has been reported for use in flow cytometric analysis.Applications Tested: The SN3 antibodyhas been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD24 is a two-chain glycosylphosphatidylinositol (GPI)-anchored glycoprotein expressed at multiple stages of B-cell development, beginning with the bone marrow pro-B-cell compartment and continuing through mature, surface Ig positive B-cells. Plasma cell expression is very low or negative. CD24 is also expressed on the majority of B-lineage acute lymphoblastic leukemias, B-cell CCLs and B-cell non-Hodgkin's lymphomas. CD24 may play a role in regulation of B-cell proliferation and maturation, andcontrol of autoimmunity.

数据

CD24 Antibody (14-0247-82) in FlowStaining of normal human peripheral blood cells with 0.25 µg of Mouse IgG1 K Isotype Control Purified (Product # 14-4714-82) (open histogram) or 0.25 µg of Anti-Human CD24 Purified) (filled histogram) followed by Anti-Mouse IgG FITC (Product # 11-4011-85). Cells in the lymphocyte gate were used for analysis.

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参考文献

1. Nature cell biologyGATA3 suppresses metastasis and modulates the tumour microenvironment by regulating microRNA-29b expression.2. OncotargetLlgl1 prevents metaplastic survival driven by epidermal growth factor dependent migration.3. Rheumatology (Oxford, England)Regulatory B cells are numerically but not functionally deficient in anti-neutrophil cytoplasm antibody-associated vasculitis.4. Molecular cancerHOXC8 regulates self-renewal, differentiation and transformation of breast cancer stem cells.5. OncotargetLlgl1 prevents metaplastic survival driven by epidermal growth factor dependent migration.

技术参数

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