CD24 Monoclonal Antibody (eBioSN3 (SN3 A5-2H10)), Super Bright 645,
货号:64-0247-42
规格:100 Tests
价格:3293
产品类型:流式抗体
品牌:eBioscience
抗原:CD24
物种:人
宿主:小鼠
抗体亚型:IgG1, kappa
克隆号:eBioSN3 (SN3 A5-2H10
荧光染料:Super Bright 645
类型: | 单抗 | 同型对照: | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Super Bright 645 |
浓度: | 5 µL/Test | 用法: | 5 µL (0.5 µg)/test(Flow) |
产品详细信息Description: The SN3 monoclonal antibody reacts with the human CD24 molecule, also known as Heat Stable Antigen (HSA). This 35-50 kDa molecule is anchored in the plasma membrane via phosphatidylinositol and is expressed by erythrocytes, thymocytes, peripheral lymphocytes and myeloid lineage. It has been reported that P-selectin (CD62P) binds to CD24.Applications Reported: This eBioSN3 (SN3 A5-2H10) antibody has been reported for use in flow cytometric analysis.Applications Tested: This eBioSN3 (SN3 A5-2H10) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Super Bright 645 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 645 nm. We recommend using a 660/20 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome.When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4 degrees C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 405 nm; Emission: 645 nm; Laser: Violet LaserSuper Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.靶标信息CD24 is a two-chain glycosylphosphatidylinositol (GPI)-anchored glycoprotein expressed at multiple stages of B-cell development, beginning with the bone marrow pro-B-cell compartment and continuing through mature, surface Ig positive B-cells. Plasma cell expression is very low or negative. CD24 is also expressed on the majority of B-lineage acute lymphoblastic leukemias, B-cell CCLs and B-cell non-Hodgkin's lymphomas. CD24 may play a role in regulation of B-cell proliferation and maturation, andcontrol of autoimmunity.
数据 |
CD24 Antibody (64-0247-42) in FlowStaining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control Super Bright 645 (Product # 64-4714) (blue histogram) or Anti-Human CD24 Super Bright 645 (purple histogram). Cells in the granulocyte gate were used for analysis. |
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